Tang Shi, Hu Yaodong, Luo Jiahui, Hu Meijun, Chen Maolin, Ye Dehan, Ye Jingsong, Xue Fuguang
College of Animal Science, Xichang University, Xichang 615000, China.
College of Animal Science, Jiangxi Agricultural University, Nanchang 330045, China.
Animals (Basel). 2024 Sep 26;14(19):2780. doi: 10.3390/ani14192780.
(1) Background: Improving feed efficiency and the vitality of the reproductive system in the late stage of the egg-laying period is of great significance for prolonging the egg-laying cycle and improving egg quality. In the present study, a new flavonoid, which was extracted from leaf, was chosen to investigate its effects on the productive performance and egg quality of late-laying hens. (2) Methods: A total of 360 500-day-old Hy-Line Brown layer hens were randomly divided into a control treatment group (no KLF supplementation), and groups that received 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% KLF supplement treatments. Each treatment contained 6 replicates, with 10 hens in each replicate. Productive performance metrics, including the daily egg production, egg weight, the number of deformed eggs, egg quality, egg density, egg shape index, eggshell strength, yolk color, and the Haugh unit, were meticulously recorded for each replicate. Furthermore, microbial communities and hypothalamus gene expressions were investigated based on the results of the productive performance and egg quality. (3) Results: KLF supplementation significantly decreased the deformity rate while significantly increasing the eggshell strength in the finishing phase afterward ( < 0.05). Specifically, hens supplemented with 0.6% KLF possessed the lowest deformed egg rate. KLF supplementation significantly increased the relative abundances of sp., sp., sp., and sp., while significantly decreasing sp. and sp. ( < 0.05). Furthermore, the interactive analysis showed the hypothalamus gene expression mainly interacted with probiotics, such as sp. and sp., through ribosome biogenesis, nucleocytoplasmic transport, and cAMP signaling pathways. (4) Conclusions: The findings of the present study indicate that KLF supplementation significantly proliferated probiotics, such as and , which may have further interacted with hypothalamus genes, thus decreasing the deformity rate while increasing eggshell strength in the finishing phase.
(1) 背景：提高产蛋后期的饲料效率和生殖系统活力对于延长产蛋周期和提高蛋品质具有重要意义。在本研究中，选取了一种从叶片中提取的新型黄酮类化合物，以研究其对产蛋后期母鸡生产性能和蛋品质的影响。(2) 方法：将总共360只500日龄的海兰褐蛋鸡随机分为一个对照处理组（不添加KLF）以及接受0.2%、0.4%、0.6%、0.8%和1.0% KLF添加处理的组。每个处理包含6个重复，每个重复中有10只母鸡。对每个重复细致记录生产性能指标，包括日产蛋量、蛋重、畸形蛋数量、蛋品质、蛋密度、蛋形指数、蛋壳强度、蛋黄颜色和哈氏单位。此外，根据生产性能和蛋品质结果研究微生物群落和下丘脑基因表达。(3) 结果：添加KLF显著降低了畸形率，同时在后期显著提高了蛋壳强度（P<0.05）。具体而言，添加0.6% KLF的母鸡畸形蛋率最低。添加KLF显著增加了[具体菌种1]、[具体菌种2]、[具体菌种3]和[具体菌种4]的相对丰度，同时显著降低了[具体菌种5]和[具体菌种6]（P<0.05）。此外，交互分析表明下丘脑基因表达主要通过核糖体生物合成、核质运输和cAMP信号通路与益生菌，如[具体菌种1]和[具体菌种2]相互作用。(4) 结论：本研究结果表明，添加KLF显著增殖了[具体菌种1]和[具体菌种2]等益生菌，这些益生菌可能进一步与下丘脑基因相互作用，从而在后期降低畸形率并提高蛋壳强度。
