Molon Mateusz, Zadrag-Tecza Renata
Department of Biochemistry and Cell Biology, University of Rzeszow, Rzeszów, Poland.
Acta Biochim Pol. 2016;63(2):329-34. doi: 10.18388/abp.2015_1164. Epub 2016 Feb 29.
The yeast Saccharomyces cerevisiae has long been used as a model organism for studying the basic mechanisms of aging. However, the main problem with the use of this unicellular fungus is the unit of "longevity". For all organisms, lifespan is expressed in units of time, while in the case of yeast it is defined by the number of daughter cells produced. Additionally, in yeast the phenotypic effects of mutations often show a clear dependence on the genetic background, suggesting the need for an analysis of strains representing different genetic backgrounds. Our results confirm the data presented in earlier papers that the reproductive potential is strongly associated with an increase in cell volume per generation. An excessive cell volume results in the loss of reproductive capacity. These data clearly support the hypertrophy hypothesis. The time of life of all analysed mutants, with the exception of sch9D, is the same as in the case of the wild-type strain. Interestingly, the 121% increase of the fob1D mutant's reproductive potential compared to the sfp1D mutant does not result in prolongation of the mutant's time of life (total lifespan).
酿酒酵母长期以来一直被用作研究衰老基本机制的模式生物。然而，使用这种单细胞真菌的主要问题在于“寿命”的单位。对于所有生物体而言，寿命是以时间单位来表示的，而对于酵母来说，它是由产生的子细胞数量来定义的。此外，在酵母中，突变的表型效应通常明显依赖于遗传背景，这表明需要对代表不同遗传背景的菌株进行分析。我们的结果证实了早期论文中所呈现的数据，即生殖潜力与每代细胞体积的增加密切相关。细胞体积过大导致生殖能力丧失。这些数据明确支持了肥大假说。除了sch9D突变体之外，所有分析的突变体的生命时长与野生型菌株相同。有趣的是，与sfp1D突变体相比，fob1D突变体的生殖潜力增加了121%，但这并未导致该突变体生命时长（总寿命）的延长。
