Molon Mateusz, Zebrowski Jacek
Department of Biochemistry and Cell Biology, University of Rzeszow, 35-601 Rzeszow, Poland.
Department of Plant Physiology, Institute of Biotechnology, University of Rzeszow, 36-100 Kolbuszawa, Poland.
FEMS Yeast Res. 2017 Jun 1;17(4). doi: 10.1093/femsyr/fox031.
Yeast ageing has been gaining much attention in gerontology research, yet the process itself is still not entirely clear. One of the constraints related to the use of the Saccharomyces cerevisiae yeast in studies is the ambiguity of the results concerning ageing determinants for different genetic backgrounds. In this paper, we compare reproductive potentials and lifespans of seven widely used haploid laboratory strains differing in daughter cells production capabilities and highlight the importance of choosing an appropriate genotype for the studies on ageing. Moreover, we show here links between post-reproductive lifespan and lipid metabolism, as well as between reproductive potential, reproductive lifespan and phylogenetic relationship. Using FTIR spectroscopy that generated a biochemical fingerprint of cells, coupled with chemometrics, we found that the band of carbonyl (C = O) stretching vibration discriminates the strains according to post-reproductive lifespan. The results indicated that prolonged post-reproductive lifespan was associated with relatively lower amount of fatty acids esterified to phospholipids compared to a free acid pool, thus implying phospholipid metabolism for the post-reproductive lifespan of yeast. In addition, phylogenetic analysis showed a correlation between nucleotide similarity and the reproductive potential or reproductive lifespan, but not to the longevity expressed in time units.
酵母衰老在老年学研究中备受关注，但其过程本身仍不完全清楚。在研究中使用酿酒酵母的一个限制因素是，对于不同遗传背景的衰老决定因素，结果存在模糊性。在本文中，我们比较了七种广泛使用的单倍体实验室菌株的繁殖潜力和寿命，这些菌株在子细胞产生能力方面存在差异，并强调了选择合适的基因型用于衰老研究的重要性。此外，我们在此展示了繁殖后期寿命与脂质代谢之间的联系，以及繁殖潜力、繁殖寿命与系统发育关系之间的联系。使用傅里叶变换红外光谱（FTIR）生成细胞的生化指纹图谱，并结合化学计量学，我们发现羰基（C = O）伸缩振动带根据繁殖后期寿命区分菌株。结果表明，与游离酸池相比，繁殖后期寿命延长与酯化到磷脂中的脂肪酸含量相对较低有关，这意味着磷脂代谢与酵母的繁殖后期寿命有关。此外，系统发育分析表明核苷酸相似性与繁殖潜力或繁殖寿命之间存在相关性，但与以时间单位表示的寿命无关。
