Chandra Saurav, Muir Eric R, Deo Kaiwalya, Kiel Jeffrey W, Duong Timothy Q
Invest Ophthalmol Vis Sci. 2016 Mar;57(3):826-31. doi: 10.1167/iovs.15-18291.
To test the hypothesis that acute topical dorzolamide (DZ) decreases intraocular pressure (IOP) and increases retinal and choroidal blood flow in the DBA/2J mouse model of glaucoma.
Retinal and choroidal blood flow were measured in 4- and 9-month-old DBA/2J mice, and 4-month C57BL/6 (control) mice under isoflurane anesthesia using magnetic resonance imaging. Ocular blood flow was measured at baseline, and 1 and 2 hours after topical dorzolamide. Intraocular pressure was measured using a rebound tonometer in a subset of animals at the same time points.
Baseline IOP in the 4-month-old DBA/2J mice and C57BL/6 mice was not significantly different (P > 0.05), and IOP in both groups was less than in the 9-month-old DBA/2J mice (P < 0.05 for both). Compared to baseline, dorzolamide reduced IOP at 1 and 2 hours after dorzolamide in the 4- (P < 0.05) and 9-month-old (P < 0.01) DBA/2J mice, but not in the C57BL/6J mice (P > 0.05). Baseline retinal blood flow was lower in the 4-month and 9-month-old DBA/2J mice compared with the 4-month-old C57BL/6J mice (P < 0.05). Baseline choroidal blood flow in the 9-month-old DBA/2J mice was less than in the C57BL/6J mice (P < 0.05). Compared with baseline, both retinal and choroidal blood flow increased at 1-hour post-dorzolamide and remained elevated 2 hours later in the 9-month-old DBA/2J mice (P < 0.05).
Dorzolamide lowers IOP and raises retinal and choroidal blood flow in older DBA/2J mice, consistent with the study hypothesis.
验证急性局部应用多佐胺（DZ）可降低青光眼DBA/2J小鼠模型的眼压（IOP）并增加视网膜和脉络膜血流这一假说。
在异氟烷麻醉下，使用磁共振成像测量4月龄和9月龄DBA/2J小鼠以及4月龄C57BL/6（对照）小鼠的视网膜和脉络膜血流。在基线、局部应用多佐胺后1小时和2小时测量眼部血流。在同一时间点，使用回弹眼压计在一部分动物中测量眼压。
4月龄DBA/2J小鼠和C57BL/6小鼠的基线眼压无显著差异（P>0.05），且两组的眼压均低于9月龄DBA/2J小鼠（两者P<0.05）。与基线相比，多佐胺使4月龄（P<0.05）和9月龄（P<0.01）DBA/2J小鼠在应用多佐胺后1小时和2小时的眼压降低，但在C57BL/6J小鼠中未降低（P>0.05）。与4月龄C57BL/6J小鼠相比，4月龄和9月龄DBA/2J小鼠的基线视网膜血流较低（P<0.05）。9月龄DBA/2J小鼠的基线脉络膜血流低于C57BL/6J小鼠（P<0.05）。与基线相比，9月龄DBA/2J小鼠在应用多佐胺后1小时视网膜和脉络膜血流均增加，并在2小时后保持升高（P<0.05）。
多佐胺可降低老龄DBA/2J小鼠的眼压并增加视网膜和脉络膜血流，与研究假说一致。
