Black J B, Sanderlin K C, Goldsmith C S, Gary H E, Lopez C, Pellett P E
Department of Experimental Pathology, Emory University, Atlanta, GA 30322.
J Virol Methods. 1989 Nov;26(2):133-45. doi: 10.1016/0166-0934(89)90143-2.
Experiments performed to optimize the growth conditions of HHV-6(Z29) revealed that the virus grows best in phytohemagglutinin-stimulated umbilical cord blood lymphocytes (CBL) cultured in media containing 32 units/ml interleukin-2 and 0.01 mg/ml hydrocortisone. The titer of maternal antibody in the plasma of the cord blood cells does not affect the ability of the cells to support virus growth. DEAE-dextran and polybrene do not increase virus growth in umbilical cord blood lymphocytes. Phorbol myristate acetate abolishes virus growth. The HHV-6(Z29) growth cycle in CBL was approximately 5 days; capsids were not seen before day 3, and mature virions were not seen before day 5.
为优化人疱疹病毒6型（Z29）（HHV-6(Z29)）的生长条件而进行的实验表明，该病毒在含有32单位/毫升白细胞介素-2和0.01毫克/毫升氢化可的松的培养基中培养的植物血凝素刺激的脐带血淋巴细胞（CBL）中生长最佳。脐带血细胞血浆中的母源抗体滴度不影响细胞支持病毒生长的能力。二乙氨基乙基葡聚糖（DEAE-葡聚糖）和聚凝胺不会增加脐带血淋巴细胞中的病毒生长。佛波酯十四烷酸酯会抑制病毒生长。CBL中HHV-6(Z29)的生长周期约为5天；在第3天之前未见衣壳，在第5天之前未见成熟病毒粒子。
