Roffman E, Frenkel N
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases/Twinbrook, National Institutes of Health, Rockville, Maryland 20852.
Virology. 1990 Apr;175(2):591-4. doi: 10.1016/0042-6822(90)90447-y.
Human herpesvirus-6 (HHV-6) is a recently identified T lymphotropic virus. We have examined the ability of HHV-6 to replicate in mature and immature human thymocytes. Infection of both cell populations revealed that only mitogen-activated mature thymocytes could support efficient virus replication. Because interleukin-2 (IL-2) plays a central role in T cell activation we investigated its effect on HHV-6 replication. Unexpectedly, addition of recombinant IL-2 at concentrations-exceeding 10 U/ml strongly inhibited the virus-induced cytopathic effect. Electron microscopic examinations and immunofluorescence assays revealed a threefold reduction in the fraction of infected cells, and almost total absence of extracellular virions in the IL-2-treated cultures. It will therefore be of interest to determine whether the IL-2-mediated inhibitory effect plays some role in the establishment of HHV-6 latency in the human host.
人类疱疹病毒6型（HHV-6）是一种最近发现的嗜T淋巴细胞病毒。我们检测了HHV-6在成熟和未成熟人胸腺细胞中的复制能力。对这两种细胞群体的感染表明，只有丝裂原激活的成熟胸腺细胞能够支持高效的病毒复制。由于白细胞介素-2（IL-2）在T细胞激活中起核心作用，我们研究了其对HHV-6复制的影响。出乎意料的是，添加浓度超过10 U/ml的重组IL-2强烈抑制了病毒诱导的细胞病变效应。电子显微镜检查和免疫荧光分析显示，感染细胞比例降低了三倍，并且在IL-2处理的培养物中几乎完全没有细胞外病毒粒子。因此，确定IL-2介导的抑制作用是否在人类宿主中HHV-6潜伏的建立中发挥某种作用将是很有意义的。
