Juarez Becerril Oswaldo, Salgado Ceballos Hermelinda, Anguiano Solis Candy, Alvarado Sanchez Belen, Lopez Hernandez Monica Elba, Diaz Ruiz Araceli, Torres Castillo Sergio
Acupunct Electrother Res. 2015;40(4):355-69.
In the present study, the effect of electro-acupuncture (EA) on the oxidative stress, the spinal cord tissue preservation and the recovery of motor function was evaluated after a traumatic spinal cord injury (TSCI). Long Evans rats were randomized into five groups: 1. Sham; 2. TSCI without treatment; 3. TSCI + EA (acupoint GV.4); 4. TSCI + EA (acupoint GV.26) and 5.TSCI + EA (GV.4 + GV.26). The EA was performed with an Electro-Acupunctoscope, AWQ-104L Digital, wave dense-dispersed, current intensity 2.5mA and frequency 2-100Hz for 30 minutes. The biochemical results showed a significant increase in the hydroxyl radical concentration in group 2 (3.1 ± 1.4 nmol) compared with groups 1 (1.8 ± 0.5 nmol) and 4 (2.4 ± 1.1 nmol) (p< 0.05), whereas in group 4 (4.8 ± 1.8 nmol), there was a significant increase in lipid peroxidation when compared with group 1 (1.7 ± 0.5 nmol) (p < 0.05). The BBB motor function score in the paralyzed hind limbs (normal BBB = 21points) was greater in groups 3 (15.2 points) and 5 (13.5 points) in comparison with groups 2 (11.4 points) and 4 (9.3 points) (p < 0.05). The quantity of preserved spinal cord tissue was greater in group 3 (6582.7± 20 μm2) than in groups 2 (5262.4 20 μm2), 4 (3995.6 ± 26μm2) and 5 (4266.7± 22 μm2). Although EA in GV.26 decreases hydroxyl radical concentration (50%), it significantly increases lipid peroxidation (45%), while stimulation of GV.4 decreases oxidative stress (15%), preserves spinal cord tissue (25%) and improves recovery of motor function in the hind limbs of rats with paralysis (18.1%) compared with untreated group. These findings suggest that EA in GV.4 may be a therapeutic alternative on TSCI.
在本研究中，评估了电针（EA）对创伤性脊髓损伤（TSCI）后氧化应激、脊髓组织保存及运动功能恢复的影响。将Long Evans大鼠随机分为五组：1. 假手术组；2. 未治疗的TSCI组；3. TSCI + EA（穴位GV.4）组；4. TSCI + EA（穴位GV.26）组和5. TSCI + EA（GV.4 + GV.26）组。使用AWQ - 104L数字式电针仪进行电针治疗，疏密波，电流强度2.5mA，频率2 - 100Hz，持续30分钟。生化结果显示，与第1组（1.8±0.5nmol）和第4组（2.4±1.1nmol）相比，第2组（3.1±1.4nmol）的羟自由基浓度显著升高（p<0.05）；而与第1组（1.7±0.5nmol）相比，第4组（4.8±1.8nmol）的脂质过氧化显著增加（p<0.05）。瘫痪后肢的BBB运动功能评分（正常BBB = 21分）在第3组（15.2分）和第5组（13.5分）中高于第2组（11.4分）和第4组（9.3分）（p<0.05）。第3组（6582.7±20μm2）保存的脊髓组织数量多于第2组（5262.4±20μm2）、第4组（3995.6±26μm2）和第5组（4266.7±22μm2）。尽管针刺GV.26可降低羟自由基浓度（50%），但显著增加脂质过氧化（45%），而针刺GV.4与未治疗组相比，可降低氧化应激（15%），保存脊髓组织（25%），并改善瘫痪大鼠后肢的运动功能恢复（18.1%）。这些发现表明，针刺GV.4可能是TSCI的一种治疗选择。
