Pacia Marta Z, Buczek Elzbieta, Blazejczyk Agnieszka, Gregorius Aleksandra, Wietrzyk Joanna, Chlopicki Stefan, Baranska Malgorzata, Kaczor Agnieszka
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.
Jagiellonian Centre of Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.
Anal Bioanal Chem. 2016 May;408(13):3381-7. doi: 10.1007/s00216-016-9436-9. Epub 2016 Mar 2.
It was recently reported in the murine model of metastatic breast cancer (4T1) that tumor progression and development of metastasis is associated with systemic endothelial dysfunction characterized by impaired nitric oxide (NO) production. Using Raman 3D confocal imaging with the analysis of the individual layers of the vascular wall combined with AFM endothelial surface imaging, we demonstrated that metastasis-induced systemic endothelial dysfunction resulted in distinct chemical changes in the endothelium of the aorta. These changes, manifested as a significant increase in the protein content (18%) and a slight decrease in the lipid content (4%), were limited to the endothelium and did not occur in the deeper layers of the vascular wall. The altered lipid to protein ratio in the endothelium, although more pronounced in the fixed vascular wall, was also observed in the freshly isolated unfixed vascular wall samples in the aqueous environment (12 and 7% change of protein and lipid content, respectively). Our results support the finding that the metastasis induces systemic endothelial dysfunction that may contribute to cancer progression.
最近在转移性乳腺癌（4T1）的小鼠模型中报道，肿瘤进展和转移的发生与以一氧化氮（NO）生成受损为特征的全身内皮功能障碍有关。通过拉曼3D共聚焦成像结合血管壁各层分析以及原子力显微镜（AFM）内皮表面成像，我们证明转移诱导的全身内皮功能障碍导致主动脉内皮发生明显的化学变化。这些变化表现为蛋白质含量显著增加（18%）和脂质含量略有下降（4%），仅限于内皮，未发生在血管壁的更深层。内皮中脂质与蛋白质比例的改变，尽管在固定的血管壁中更为明显，但在水性环境中新鲜分离的未固定血管壁样本中也观察到了（蛋白质和脂质含量分别变化12%和7%）。我们的结果支持以下发现：转移诱导全身内皮功能障碍，这可能促进癌症进展。
