Pascual Javier, González Ignacio, Estévez Mar, Benito Patricia, Trujillo Martha E, Genilloud Olga
Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avenida del Conocimiento, 34. Parque Tecnológico de Ciencias de la Salud, 18016, Granada, Spain.
Department of Microbial Ecology and Diversity Research, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7 B, 38124, Brunswick, Germany.
Antonie Van Leeuwenhoek. 2016 May;109(5):685-95. doi: 10.1007/s10482-016-0669-6. Epub 2016 Mar 2.
A novel actinomycete strain F-240,109(T) from the MEDINA collection was isolated from a soil sample collected in the forest of Pama, on the plateau of Bangui, Central African Republic. The strain was identified according to its 16S rRNA gene sequence as a new member of the genus Kibdelosporangium, being closely related to Kibdelosporangium aridum subsp. aridum (98.6 % sequence similarity), Kibledosporangium phytohabitans (98.3 %), Kibdelosporangium aridum subsp. largum (97.7 %), Kibdelosporangium philippinense (97.6 %) and Kibledosporangium lantanae (96.9 %). In order to resolve its precise taxonomic status, the strain was characterised through a polyphasic approach. The strain is a Gram-stain positive, aerobic, non-motile and catalase-positive actinomycete characterised by formation of extensively branched substrate mycelia and sparse brownish grey aerial mycelia with sporangium-like globular structures. The chemotaxonomic characterisation of strain F-240,109(T) corroborated its affiliation into the genus Kibdelosporangium. The peptidoglycan contains meso-diaminopimelic acid; the major menaquinone is MK-9(H4); the phospholipid profile contains high amounts of phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol and an unidentified phospholipid; and the predominant cellular fatty acid methyl esters are iso-C16:0, iso-C14:0, iso-C15:0 and 2OH iso-C16:0. However, some key phenotypic differences regarding to its close relatives and DNA-DNA hybridization values indicate that strain F-240,109(T) represents a novel Kibdelosporangium species, for which the name Kibdelosporangium banguiense sp. nov. is proposed. The type strain is strain F-240,109(T) (=DSM 46670(T), =LMG 28181(T)).
从位于中非共和国班吉高原帕马森林采集的土壤样本中，分离出一株来自MEDINA菌种保藏库的新型放线菌菌株F-240,109(T)。根据其16S rRNA基因序列，该菌株被鉴定为基德孢囊菌属的一个新成员，与干旱基德孢囊菌干旱亚种（序列相似性98.6%）、栖植物基德孢囊菌（98.3%）、干旱基德孢囊菌大孢亚种（97.7%）、菲律宾基德孢囊菌（97.6%）和马缨丹基德孢囊菌（96.9%）密切相关。为确定其精确的分类地位，采用多相分类法对该菌株进行了表征。该菌株是革兰氏染色阳性、需氧、不运动且过氧化氢酶阳性的放线菌，其特征在于形成广泛分支的基内菌丝体和带有类孢子囊球状结构的稀疏棕灰色气生菌丝体。菌株F-240,109(T)的化学分类特征证实了它属于基德孢囊菌属。肽聚糖含有内消旋二氨基庚二酸；主要甲基萘醌是MK-9(H4)；磷脂谱含有大量的磷脂酰乙醇胺、羟基磷脂酰乙醇胺、二磷脂酰甘油和一种未鉴定的磷脂；主要的细胞脂肪酸甲酯是异-C16:0、异-C14:0、异-C15:0和2-OH异-C16:0。然而，与它的近缘种相比，一些关键的表型差异以及DNA-DNA杂交值表明菌株F-240,109(T)代表一个新的基德孢囊菌物种，为此提出新名称班吉基德孢囊菌（Kibdelosporangium banguiense sp. nov.）。模式菌株是菌株F-240,109(T)（=DSM 46670(T)，=LMG 28181(T)）。
