Microbial Strain Collection, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany.
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Inhoffenstrasse 7B, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004625.
Isolate 4NS15 was isolated from a neglected arid habitat in Kerman, Iran. The strain showed 16S rRNA gene sequence similarity values of 98.9 % to the type strains of subsp. , and and 98.6 % to the type strain subsp. , respectively. Genome-based phylogenetic analysis revealed that isolate 4NS15 is closely related to subsp. DSM 43828. The digital DNA-DNA hybridization value between the genome sequences of 4NS15 and strain DSM 43828 is 29.8 %. Strain 4NS15 produces long chains of spores without a sporangium-like structure which can be distinguished from other species. Isolate 4NS15 has a genome size of 10.35 Mbp with a G+C content of 68.1 mol%. Whole-cell hydrolysates of isolate 4NS15 are rich in -diaminopimelic acid and cell-wall sugars such as arabinose, galactose, glucose and ribose. Major fatty acids (>10 %) are C, iso-C and iso-C. The phospholipid profile contains diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylhydroxyethanolamine, aminolipid and glycoaminolipid. The predominant menaquinone is MK-9(H). Based on its phenotypic and genotypic characteristics, isolate 4NS15 (NCCB 100701=CIP 111705=DSM 110728) merits recognition as representing a novel species of the genus , for which the name sp. nov. is proposed.
4NS15 菌株从伊朗克尔曼省被忽视的干旱生境中分离得到。该菌株与 subsp. 、 和 subsp. 的模式菌株的 16S rRNA 基因序列相似性值分别为 98.9%、98.6%和 98.6%。基于基因组的系统发育分析表明，分离株 4NS15 与 subsp. DSM 43828 密切相关。4NS15 与菌株 DSM 43828 基因组序列之间的数字 DNA-DNA 杂交值为 29.8%。菌株 4NS15 产生没有类似于孢子囊结构的长链孢子，可以与其他 物种区分开来。分离株 4NS15 的基因组大小为 10.35 Mbp，G+C 含量为 68.1 mol%。分离株 4NS15 的全细胞水解物富含 -二氨基庚二酸和细胞壁糖，如阿拉伯糖、半乳糖、葡萄糖和核糖。主要脂肪酸 (>10%)为 C、iso-C 和 iso-C。磷脂谱含有双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰羟乙醇胺、胺脂和糖胺脂。主要的menaquinone 是 MK-9(H)。基于其表型和基因型特征，分离株 4NS15（NCCB 100701=CIP 111705=DSM 110728）被认为代表了一个新的 属种，因此建议使用 sp. nov. 作为该新种的名称。
