School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou, Jiangsu, 221116, People's Republic of China.
Antonie Van Leeuwenhoek. 2012 Feb;101(2):433-41. doi: 10.1007/s10482-011-9652-4. Epub 2011 Oct 12.
A novel actinomycete, designated strain KLBMP 1111(T), was isolated from the root of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Strain KLBMP 1111(T) formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus Kibdelosporangium, with the highest similarity to Kibdelosporangium aridum subsp. aridum DSM 43828(T) (98.8%), K. aridum subsp. largum DSM 44150(T) (98.1%) and Kibdelosporangium philippinense DSM 44226(T) (98.1%). The organism produced sporangium-like structures, the typical morphological characteristic of the genus Kibdelosporangium. The chemotaxonomic properties of this strain were also consistent with those of the genus Kibdelosporangium: the peptidoglycan contained meso-diaminopimelic acid; the predominant menaquinone was MK-9(H(4)); phospholipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol and an unknown phospholipid; iso-C(16:0), C(16:0), anteiso-C(15:0) and iso-C(15:0) as the predominant cellular fatty acids and the G+C content was 67.2 mol%. DNA-DNA hybridization values between strain KLBMP 1111(T) and the three Kibdelosporangium species were less than 50%. This strain had the ability to produce a siderophore, utilized 1-aminocyclopropane-1-carboxylic acid (ACC) as sole source of nitrogen and possessed ACC deaminase enzyme. Based on genotypic and phenotypic data, strain KLBMP 1111(T) represents a novel species in the genus Kibdelosporangium. We propose the name Kibdelosporangium phytohabitans sp. nov. for this species. The type strain is the strain KLBMP 1111(T) (=KCTC 19775(T) = CCTCC AA 2010001(T)).
一株新的放线菌，命名为 KLBMP 1111(T) 菌株，从中国西南地区四川省收集的麻疯树植物的根部分离得到。KLBMP 1111(T) 菌株在 16S rRNA 基因系统发育树中与 Kibdelosporangium 属的模式菌株形成一个明显的分支，与 Kibdelosporangium aridum subsp. aridum DSM 43828(T)（98.8%）、K. aridum subsp. largum DSM 44150(T)（98.1%）和 Kibdelosporangium philippinense DSM 44226(T)（98.1%）的相似度最高。该菌产生类似于孢子囊的结构，这是 Kibdelosporangium 属的典型形态特征。该菌株的化学生物分类特征也与 Kibdelosporangium 属一致：肽聚糖含有 meso-二氨基庚二酸；主要的menaquinone 是 MK-9(H(4))；磷脂为磷脂酰甘油、磷脂酰乙醇胺、磷脂甲基乙醇胺、磷脂酰肌醇和一种未知的磷脂；细胞脂肪酸的主要成分是 iso-C(16:0)、C(16:0)、anteiso-C(15:0)和 iso-C(15:0)；G+C 含量为 67.2 mol%。KLBMP 1111(T) 菌株与三种 Kibdelosporangium 种之间的 DNA-DNA 杂交值小于 50%。该菌株具有产生铁载体的能力，利用 1-氨基环丙烷-1-羧酸 (ACC) 作为唯一氮源，并具有 ACC 脱氨酶酶。基于基因型和表型数据，KLBMP 1111(T) 菌株代表 Kibdelosporangium 属的一个新种。我们提议将这种新种命名为植物生 Kibdelosporangium 。该模式菌株是 KLBMP 1111(T) 菌株（=KCTC 19775(T) = CCTCC AA 2010001(T)）。
