Jang HyunSuk, Yoon Joohwan, Gil HyunJi, Jung Sharon Jiyoon, Kim Min-Suk, Lee Jin-Kyu, Kim Young-Jae, Soh Kwang-Sup
Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, 443-270, Korea.
College of Physical Education, University of Suwon, Hwaseong, 445-743, Korea.
PLoS One. 2016 Mar 3;11(3):e0150423. doi: 10.1371/journal.pone.0150423. eCollection 2016.
The primo vascular system (PVS) is being established as a circulatory system that corresponds to acupuncture meridians. There have been two critical questions in making the PVS accepted as a novel liquid flowing system. The first one was directly to show the flow of liquid in PVS and the second one was to explain why it was not observed in the conventional histological study of animal tissues. Flow in the PVS in the abdominal cavity was previously verified by injecting Alcian blue into a primo node. However, the tracing of the dye to other subsystems of the PVS has not been done. In the current work we injected fluorescent nanoparticles (FNPs) into a primo node and traced them along a primo vessel which was inside a fat tissue in the abdominal wall. Linea alba is a white middle line in the abdominal skin of a mammal and a band of fat tissue is located in parallel to the linea alba in the parietal side of the abdominal wall of a rat. In this fat band a primo vessel runs parallel to the prominent blood vessels in the fat band and is located just inside the parietal peritoneum. About the second question on the reason why the PVS was not in conventional histological study the current work provided the answer. Histological analysis with hematoxyline and eosine, Masson's trichrome, and Toluidine blue could not discriminate the primo vessel even when we knew the location of the PVS by the trace of the FNPs. This clearly explains why the PVS is hard to observe in conventional histology: it is not a matter of resolution but the contrast. The PVS has very similar structure to the connective tissues that surround the PVS. In the current work we propose a method to find the PVS: Observation of mast cell distribution with toluidine blue staining and the PN has a high density of mast cells, while the lymph node has low density.
原血管系统（PVS）正被确立为一种与针灸经络相对应的循环系统。在使PVS被接受为一种新型液体流动系统方面存在两个关键问题。第一个问题是直接展示PVS中液体的流动，第二个问题是解释为什么在动物组织的传统组织学研究中没有观察到它。腹腔内PVS中的流动先前已通过将阿尔新蓝注入原结得到证实。然而，尚未对染料在PVS其他子系统中的追踪进行研究。在当前的工作中，我们将荧光纳米颗粒（FNP）注入原结，并沿着腹壁脂肪组织内的一条原血管对其进行追踪。白线是哺乳动物腹部皮肤中的一条白色中线，在大鼠腹壁的壁侧，一条脂肪组织带与白线平行。在这条脂肪带中，一条原血管与脂肪带中突出的血管平行，且位于腹膜壁层的内侧。关于PVS为何未在传统组织学研究中被发现的第二个问题，当前的工作给出了答案。用苏木精和伊红、马松三色染色法以及甲苯胺蓝进行的组织学分析，即使我们通过FNP的追踪知道了PVS的位置，也无法区分原血管。这清楚地解释了为什么PVS在传统组织学中难以观察到：这不是分辨率的问题，而是对比度的问题。PVS与围绕它的结缔组织结构非常相似。在当前的工作中，我们提出了一种找到PVS的方法：用甲苯胺蓝染色观察肥大细胞分布，原结有高密度的肥大细胞，而淋巴结的密度较低。
