Lim Chae Jeong, Lee So Yeong, Ryu Pan Dong
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
Evid Based Complement Alternat Med. 2015;2015:751937. doi: 10.1155/2015/751937. Epub 2015 Aug 25.
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.
初级血管系统（PVS）是在各种动物组织中发现的一种新型网络。然而，皮下组织中的PVS尚未得到很好的鉴定。在这里，我们检查了大鼠腹部皮下组织表面的假定PVS。苏木精-伊红染色显示出由节点和血管组成的深蓝色丝状结构，经常观察到它们与血管束在一起。该结构充满了包括肥大细胞和白细胞在内的各种免疫细胞。在该结构中，存在细胞密度较低的内部空间（20-60μm）。电子显微镜显示出一种束状结构和与成熟的器官表面PVS相同的典型细胞学特征，这与淋巴管不同。在大鼠腹部空间中鉴定出的几种皮下（sc）PVS组织中，最突出的是沿腹中线排列的scPVS。scPVS中节点和血管的分布模式与任脉经络及其穴位的分布模式非常相似。总之，我们的结果首次揭示PVS存在于腹部皮下组织层中，并表明scPVS组织与经络密切相关。我们的发现将有助于阐明其他浅表组织中的PVS及其生理作用。
