Wojtkowiak-Giera Agnieszka, Derda Monika, Kosik-Bogacka Danuta, Kolasa-Wołosiuk Agnieszka, Wandurska-Nowak Elżbieta, Jagodziński Paweł P, Hadaś Edward
Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 10 Fredry Street, 61-701, Poznan, Poland.
Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 10 Fredry Street, 61-701, Poznan, Poland.
Exp Parasitol. 2019 Apr;199:24-29. doi: 10.1016/j.exppara.2019.02.011. Epub 2019 Feb 20.
The genus Acanthamoeba, which may cause different infections in humans, occurs widely in the environment. Lung inflammation caused by these parasites induces pulmonary pathological changes such as pulmonary necrosis, peribronchial plasma cell infiltration, moderate desquamation of alveolar cells and partial destruction of bronchial epithelial cells, and presence of numerous trophozoites and cysts among inflammatory cells. The aim of this study was to assess the influence of plant extracts from Artemisia annua L. on expression of the toll-like receptors TLR2 and TLR4 in lungs of mice with acanthamoebiasis. A. annua, which belongs to the family Asteraceae, is an annual plant that grows wild in Asia. In this study, statistically significant changes of expression of TLR2 and TLR4 were demonstrated. In the lungs of infected mice after application of extract from A. annua the expression of TLRs was observed mainly in bronchial epithelial cells, pneumocytes (to a lesser extent during the outbreak of infection), and in the course of high general TLR expression. TLR4 in particular was also visible in stromal cells of lung parenchyma. In conclusion, we confirmed that a plant extract of A. annua has a modulatory effect on components of the immune system such as TLR2 and TLR4.
棘阿米巴属可导致人类不同感染，在环境中广泛存在。这些寄生虫引起的肺部炎症会诱发肺部病理变化，如肺坏死、支气管周围浆细胞浸润、肺泡细胞中度脱落和支气管上皮细胞部分破坏，以及炎症细胞中存在大量滋养体和包囊。本研究的目的是评估黄花蒿植物提取物对棘阿米巴病小鼠肺组织中Toll样受体TLR2和TLR4表达的影响。黄花蒿属于菊科，是一种在亚洲野生生长的一年生植物。在本研究中，证实了TLR2和TLR4表达有统计学意义的变化。在应用黄花蒿提取物后，感染小鼠的肺组织中，TLR的表达主要见于支气管上皮细胞、肺细胞（在感染爆发期间程度较轻），且在总体TLR高表达过程中可见。特别是TLR4在肺实质的基质细胞中也可见。总之，我们证实黄花蒿植物提取物对免疫系统成分如TLR2和TLR4具有调节作用。
