Birlik Muhammet, Babacan Hasan, Cevit Ruhiye, Gürler Birol
Department of Orthodontics, Faculty of Dentistry, Bezmialem Vakıf University, Fatih, 34093, Istanbul, Turkey.
Department of Orthodontics, Faculty of Dentistry, Pamukkale University, Denizli, Turkey.
J Orofac Orthop. 2016 Mar;77(2):94-103. doi: 10.1007/s00056-016-0021-9. Epub 2016 Mar 4.
The purpose of this work was to evaluate the effects of sex steroids on bone formation in response to midpalatal suture expansion by means of histological and immunohistochemical examinations and computed tomography (CT).
A total of 32 male and 32 female 12-week-old Wistar rats were divided into four groups per gender. Testosterone was administered to the castrated experimental male rats, estrogen to castrated experimental female rats. Saline solution was given subcutaneously to the male and female control, sham, and castration groups during expansion and retention periods, which lasted 7 and 5 days, respectively. The expansion amount was measured with a digital caliper. The density of the new bone in the expansion area was measured via CT.
Histological and CT evaluation revealed that the number of osteoblasts and density of the new bone was higher in male and female experimental groups than in all the other groups. When scores of staining intensity were compared, the experimental groups demonstrated statistically significant greater immunoreactivity in the osteoblasts compared to castrated-only groups. Bone density was higher in the female experimental group than in the others, and higher in the male experimental group than in the others. Expansion amounts in the castrated groups were higher than in the others (p < 0.05).
Raising the levels of sex steroids in rats revealed positive effects on bone formation in the midpalatal suture in response to expansion. Increased sex steroid levels can reduce the time needed for retention.
本研究旨在通过组织学、免疫组织化学检查及计算机断层扫描（CT）评估性类固醇对腭中缝扩展后骨形成的影响。
将32只12周龄雄性和32只雌性Wistar大鼠按性别各分为四组。对去势的实验雄性大鼠给予睾酮，对去势的实验雌性大鼠给予雌激素。在分别持续7天和5天的扩展期和维持期，对雄性和雌性对照组、假手术组及去势组皮下注射生理盐水。用数字卡尺测量扩展量。通过CT测量扩展区域新骨的密度。
组织学和CT评估显示，雄性和雌性实验组的成骨细胞数量和新骨密度高于所有其他组。比较染色强度评分时，实验组的成骨细胞免疫反应性与仅去势组相比具有统计学意义的显著增加。雌性实验组的骨密度高于其他组，雄性实验组的骨密度也高于其他组。去势组的扩展量高于其他组（p < 0.05）。
提高大鼠体内性类固醇水平对腭中缝扩展后的骨形成有积极影响。性类固醇水平升高可减少维持所需时间。
