离子淌度分离肽同量异位素。
Ion Mobility Separation of Peptide Isotopomers.
机构信息
Department of Chemistry, Wichita State University, 1845 Fairmount, Wichita, KS, 67260-0051, USA.
出版信息
J Am Soc Mass Spectrom. 2016 May;27(5):795-9. doi: 10.1007/s13361-016-1367-3. Epub 2016 Mar 4.
Abstract
Differential or field asymmetric waveform ion mobility spectrometry (FAIMS) operating at high electric fields fully resolves isotopic isomers for a peptide with labeled residues. The naturally present isotopes, alone and together with targeted labels, also cause spectral shifts that approximately add for multiple heavy atoms. Separation qualitatively depends on the gas composition. These findings may enable novel strategies in proteomic and metabolomic analyses using stable isotope labeling.
摘要
在高电场下工作的差分或场非对称波形离子迁移谱（FAIMS）可以完全解析带有标记残基的肽的同位素异构体。单独存在的天然同位素以及与目标标记一起，也会引起光谱移动，这些移动大约会在多个重原子上相加。分离的质量取决于气体成分。这些发现可能会为使用稳定同位素标记的蛋白质组学和代谢组学分析提供新的策略。
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2
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