Park Sooyeon, Jung Yong-Taek, Park Ji-Min, Kim Song-Gun, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, South Korea.
University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
Int J Syst Evol Microbiol. 2016 Jun;66(6):2146-2151. doi: 10.1099/ijsem.0.001000. Epub 2016 Mar 4.
A Gram-stain-negative, aerobic, non-flagellated, non-gliding and ovoid or rod-shaped bacterium, designated DB-3T, was isolated from a tidal flat on the Yellow Sea in South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain DB-3T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain DB-3T fell within the clade comprising the type strains of species of the genus Psychroflexus. Strain DB-3T exhibited 16S rRNA gene sequence similarities of 93.2-96.9 % to the type strains of species of the genus Psychroflexus. Strain DB-3T contained MK-6 as the predominant menaquinone and iso-C15:0, anteiso-C15:0 and iso-C17:0 3-OH as the major fatty acids. The major or significant amounts of polar lipids detected in strain DB-3T were phosphatidylethanolamine, an unidentified aminolipid and seven unidentified lipids. The DNA G+C content was 34.7 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, revealed that strain DB-3T is separated from recognized species of the genus Psychroflexus. On the basis of the data presented, strain DB-3T is considered to represent a novel species of the genus Psychroflexus, for which the name Psychroflexus aestuariivivens sp. nov. is proposed. The type strain is DB-3T (=KCTC 52037T=NBRC 111757T).
从韩国黄海的潮滩中分离出一株革兰氏阴性、需氧、无鞭毛、不滑动、卵形或杆状的细菌，命名为DB-3T，并采用多相分类法对其进行分类学研究。菌株DB-3T在30°C、pH 7.0 - 8.0以及2.0 - 3.0%（w/v）NaCl条件下生长最佳。基于16S rRNA基因序列构建的系统发育树表明，菌株DB-3T属于嗜冷弯曲菌属物种模式菌株所在的进化分支。菌株DB-3T与嗜冷弯曲菌属物种模式菌株的16S rRNA基因序列相似性为93.2 - 96.9%。菌株DB-3T以MK-6作为主要甲基萘醌，以异-C15:0、anteiso-C15:0和异-C17:0 3-OH作为主要脂肪酸。在菌株DB-3T中检测到的主要或大量极性脂质为磷脂酰乙醇胺、一种未鉴定的氨基脂质和七种未鉴定的脂质。DNA G+C含量为34.7 mol%。不同的表型特征以及系统发育独特性表明，菌株DB-3T与嗜冷弯曲菌属已确认的物种不同。根据所提供的数据，菌株DB-3T被认为代表嗜冷弯曲菌属的一个新物种，为此提出新名称嗜冷潮滩弯曲菌（Psychroflexus aestuariivivens sp. nov.）。模式菌株为DB-3T（=KCTC 52037T=NBRC 111757T）。
