CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
Mar Drugs. 2019 Feb 20;17(2):124. doi: 10.3390/md17020124.
Microalgae of the genus show great potential for large-scale commercial cultivation, as they accumulate large quantities of B-phycoerythrin (B-PE), long chain polyunsaturated fatty acids (LC-PUFAs) and exopolysaccharide (EPS). The present study aimed to adjust culture nitrogen concentrations to produce biomass rich in B-PE, LC-PUFAs and EPS. SCS-02 was cultured in ASW culture medium with low nitrogen supply (LN, 3.5 mM), medium nitrogen supply (MN, 5.9 mM) or high nitrogen supply (HN, 17.6 mM). HN significantly enhanced the accumulation of biomass, intracellular protein, B-PE and eicosapentaenoic acid. LN increased the intracellular carbohydrate and arachidonic acid content, and promoted the secretion of EPS. The total lipids content was almost unaffected by nitrogen concentration. Based on these results, a semi-continuous two-step process was proposed, which included the production of biomass rich in B-PE and LC-PUFAs with sufficient nitrogen, and induced EPS excretion with limited nitrogen and strong light.
微藻属在大规模商业培养方面具有巨大的潜力，因为它们可以积累大量的藻红蛋白（B-PE）、长链多不饱和脂肪酸（LC-PUFAs）和胞外多糖（EPS）。本研究旨在调整培养氮浓度，以生产富含 B-PE、LC-PUFAs 和 EPS 的生物量。SCS-02 在低氮供应（LN，3.5 mM）、中氮供应（MN，5.9 mM）或高氮供应（HN，17.6 mM）的 ASW 培养基中培养。HN 显著促进了生物量、细胞内蛋白质、B-PE 和二十碳五烯酸的积累。LN 增加了细胞内碳水化合物和花生四烯酸的含量，并促进了 EPS 的分泌。总脂质含量几乎不受氮浓度的影响。基于这些结果，提出了一种半连续两步法，包括在充足氮的条件下生产富含 B-PE 和 LC-PUFAs 的生物量，以及在有限氮和强光条件下诱导 EPS 排泄。
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