Centre for Sustainable Aquatic Research (CSAR), Swansea University, Swansea SA2 8PP, United Kingdom.
Centre for Complex Fluids Processing, Multidisciplinary Nanotechnology Centre, College of Engineering, Swansea University, Swansea SA2 8PP, United Kingdom.
Bioresour Technol. 2015;189:357-363. doi: 10.1016/j.biortech.2015.04.042. Epub 2015 Apr 20.
The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N). FTIR was used technology to characterise macromolecule biomass composition and the quality of the metabolites produced. Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1). FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
该文化策略（分批或半连续）评估了在高纬度地区（>50°N）的紫球藻培养物中的生物量和代谢产物的形成。傅里叶变换红外光谱（FTIR）技术用于表征生物量的大分子组成和产生的代谢产物的质量。由于半连续培养在生长速率（0.27 天（-1））、倍增时间（2.5-4 天）、达到的最高细胞密度（1.43*10（7）细胞 mL（-1））、生物量生产力（47.04 mg L（-1）天（-1）和胞外多糖产量（2.1 g L（-1））方面的平均结果，因此被认为是在高纬度地区开发微藻生物量生产设施的最可行策略。傅里叶变换红外光谱技术应用于微藻生产是一种有价值且可靠的工具，可用于确定生物量组成（脂质、碳水化合物和蛋白质）的演变，还可用于表征紫球藻培养物中产生的代谢产物，如藻红蛋白或胞外多糖。
