Hajimiri Elahe Sadat, Masoomi Morteza, Ebrahimzadeh Nayereh, Fateh Abolfazl, Hadizadeh Tasbiti Alireza, Rahimi Jamnani Fatemeh, Bahrmand Ahmad Reza, Mirsaeidi Mehdi, Vaziri Farzam, Siadat Seyed Davar
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.
Microb Pathog. 2016 Apr;93:213-8. doi: 10.1016/j.micpath.2016.02.015. Epub 2016 Mar 2.
Recent studies using molecular epidemiological techniques have demonstrated mixed infection with multiple strains of Mycobacterium tuberculosis especially in countries with high tuberculosis (TB) burden. We aimed to determine the prevalence of mixed infection among patients with TB in the capital of Iran as a country with moderate incidence rate.
Samples were collected randomly from January 2011 to December 2013 in Tehran, capital of Iran. A total of 75 M. tuberculosis isolates were genotyped by 24 loci mycobacterial interspersed repetitive unit-variable number tandem repeat typing (MIRU-VNTR) for screening the mixed infection.
Twenty patients (20/75) were identified with mixed infection, and the estimated rate of mixed infection was 26.6%. Thirteen out of the 24 loci were able to detect the mixed infection in our study.
Mixed infections occur at high prevalence among studied Iranian TB patients. Further research is inevitable to evaluate the association of mixed infection and disease progression and treatment.
近期使用分子流行病学技术的研究表明，结核分枝杆菌多菌株混合感染情况普遍，尤其是在结核病负担较高的国家。作为一个发病率中等的国家，我们旨在确定伊朗首都结核病患者中混合感染的患病率。
2011年1月至2013年12月期间，在伊朗首都德黑兰随机采集样本。通过24个位点的分枝杆菌插入重复单位-可变数目串联重复序列分型法（MIRU-VNTR）对总共75株结核分枝杆菌分离株进行基因分型，以筛查混合感染情况。
20例患者（20/75）被鉴定为混合感染，混合感染估计率为26.6%。在我们的研究中，24个位点中的13个能够检测到混合感染。
在所研究的伊朗结核病患者中，混合感染的发生率很高。进一步研究不可避免，以评估混合感染与疾病进展及治疗之间的关联。
