伊朗西南部铜绿假单胞菌分离株中 blaPER-1 和 blaVEB-1β-内酰胺酶基因的存在。
Presence of blaPER-1 and blaVEB-1 beta-lactamase genes among isolates of Pseudomonas aeruginosa from South West of Iran.
机构信息
Department of Microbiology, School of Paramedical Sciences, Ilam University of Medical Sciences, Ilam, Iran.
Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
出版信息
J Epidemiol Glob Health. 2016 Sep;6(3):211-3. doi: 10.1016/j.jegh.2016.02.002. Epub 2016 Mar 2.
Pseudomonas aeruginosa isolates have acquired resistance to antibiotics such as novel beta-lactams. The aim of this study was to investigate the blaPER-1, blaVEB-1, and blaPSE-1 genes among isolates of P. aeruginosa among intensive care unit (ICU) patients. Sixty-five isolates were collected. The antibiotic susceptibility testing and combined disk tests were performed to detect the isolates producing extended spectrum beta-lactamases (ESBLs) among ceftazidime-resistant isolates. Polymerase chain reaction (PCR) amplification of blaPER-1, blaVEB-1, and blaPSE-1 genes was conducted. Ten (15.3%) isolates were ESBL-positive, of which 40% (n=4) belonged to males and 60% (n=6) were collected from females. Moreover, two and one isolates harbored blaPER-1 and blaVEB-1 genes, respectively.
铜绿假单胞菌分离株已获得对新型β-内酰胺类抗生素的耐药性。本研究旨在调查重症监护病房（ICU）患者中铜绿假单胞菌分离株的 blaPER-1、blaVEB-1 和 blaPSE-1 基因。共采集了 65 株分离株。对头孢他啶耐药的分离株进行了抗生素敏感性试验和联合药敏试验，以检测产超广谱β-内酰胺酶（ESBLs）的分离株。采用聚合酶链反应（PCR）扩增 blaPER-1、blaVEB-1 和 blaPSE-1 基因。10 株（15.3%）分离株为 ESBL 阳性，其中 40%（n=4）为男性，60%（n=6）为女性。此外，有 2 株和 1 株分离株分别携带 blaPER-1 和 blaVEB-1 基因。
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