益生菌形态对肉鸡生长性能、消化功能及免疫相关生物标志物的影响。

Probiotic form effects on growth performance, digestive function, and immune related biomarkers in broilers.

作者信息

Palamidi I, Fegeros K, Mohnl M, Abdelrahman W H A, Schatzmayr G, Theodoropoulos G, Mountzouris K C

机构信息

Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Iera Odos 75, 11855, Greece.

BIOMIN Holding GmbH, Erber Campus 1, 3131 Getzersdorf, Austria.

出版信息

Poult Sci. 2016 Jul 1;95(7):1598-1608. doi: 10.3382/ps/pew052. Epub 2016 Mar 4.

DOI:10.3382/ps/pew052

PMID:26944970

Abstract

The aim of this work was to assess the effect of dietary viable or heat inactivated probiotic forms (PF) combined or not with avilamycin (AV) used as a growth promoter, on broiler growth performance, nutrient digestibility, digestive enzyme activities, and expression of immune response related genes.Depending on the type of PF (i.e., no addition, viable, inactivated) and AV addition (no/yes), 450 one-day-old Cobb male broilers were allocated in the following 6 treatments according to a 3 × 2 factorial arrangement with 5 replicates of 15 broilers each for 6 wk: CoN: diet without any addition; CoN+A: combination of CoN with AV; ViP: viable PF - no AV; ViP+A: combination of ViP with AV; InP: inactivated PF - no AV; InP+A: combination of InP with AV.There were no interactions (P > 0.05) for overall performance parameters. In contrast, PF or AV addition improved BW gain (PPF= 0.015; PAV < 0.001), FCR (PPF < 0.001; PAV < 0.001) and production efficiency factor (PPF= 0.001; PAV= 0.001).Significant (PPF×AV ≤ 0.05) interaction effects regarding ileal digestibility (IAD) of DM and total tract apparent digestibility (TTAD) of DM and ether extracts (EE) were noted. In addition, PF affected IAD and TTAD of CP (PPF < 0.001, PPF= 0.004, respectively). Inactivated PF increased (PPR= 0.024) lipase activity in jejunal digesta.At spleen level InP and ViP+A down-regulated TGF-β4 (PPF × AV = 0.035) compared to CoN and ViP, whereas ViP+A up-regulated iNOS (PPF × AV = 0.022). An anti-inflammatory effect of live and inactive PF and/or AV addition at cecal tonsils was shown by iNOS down-regulation (PPF × AV= 0.015) compared to CoN. Furthermore, AV down-regulated IFN-γ (PAV= 0.002).In conclusion, viable probiotic, as well as inactivated probiotic alone or in combination with avilamycin, improved nutrient digestibility. All dietary additives affected growth performance positively and induced an anti-inflammatory response at cecal level.

摘要

本研究旨在评估日粮中添加或不添加作为生长促进剂的阿维拉霉素（AV）的活的或热灭活的益生菌形式（PF）对肉鸡生长性能、养分消化率、消化酶活性以及免疫反应相关基因表达的影响。根据PF的类型（即不添加、活的、灭活的）和AV的添加情况（不添加/添加），将450只1日龄的科宝雄性肉鸡按照3×2析因设计分为以下6个处理组，每组15只肉鸡，重复5次，试验期为6周：对照组（CoN）：不添加任何物质的日粮；CoN+A组：CoN与AV的组合；活的PF组（ViP）：活的PF，不添加AV；ViP+A组：ViP与AV的组合；灭活的PF组（InP）：灭活的PF，不添加AV；InP+A组：InP与AV的组合。总体生产性能参数不存在交互作用（P>0.05）。相比之下，添加PF或AV可提高体重增加（PPF=0.015；PAV<0.001）、饲料转化率（PPF<0.001；PAV<0.001）和生产效率因子（PPF=0.001；PAV=0.001）。观察到关于干物质（DM）的回肠消化率（IAD）以及DM和乙醚提取物（EE）的全肠道表观消化率（TTAD）存在显著的（PPF×AV≤0.05）交互作用。此外，PF影响CP的IAD和TTAD（PPF分别<0.001和=0.004）。灭活的PF增加了空肠食糜中的脂肪酶活性（PPR=0.024）。在脾脏水平，与CoN和ViP相比，InP和ViP+A下调了TGF-β4（PPF×AV=0.035），而ViP+A上调了iNOS（PPF×AV=0.022）。与CoN相比，在盲肠扁桃体处添加活的和灭活的PF和/或AV显示出通过下调iNOS产生的抗炎作用（PPF×AV=0.015）。此外，AV下调了IFN-γ（PAV=0.002）。总之，活的益生菌以及单独或与阿维拉霉素联合使用的灭活益生菌均可提高养分消化率。所有日粮添加剂均对生长性能产生积极影响，并在盲肠水平诱导抗炎反应。