College of Animal Sciences, Zhejiang University, Hangzhou, China.
The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, China.
J Anim Sci. 2024 Jan 3;102. doi: 10.1093/jas/skae133.
Adding multienzymes to poultry feed rations is recognized as a nutritional strategy aimed at improving poultry performance and health status. Nonetheless, some literatures present an ongoing debate about the extent of multienzymes beneficial impact on poultry growth performance. This study aimed to explore the impacts of dietary multienzyme supplementation on broilers, focusing specifically on growth performance, carcass characteristics, apparent nutrient digestibility, excreta noxious gas emission, and intestinal nutrient transporter gene expression. A total of 3,200 broilers were randomly assigned to five groups (eight replicates per treatment group) and treated with the following: normal control (CON), CON + 100 g/t multienzyme (ME100), CON + 150 g/t multienzyme (ME150), CON + 200 g/t multienzyme (ME200), and CON + 250 g/t multienzyme (ME250). Supplementing with multienzymes significantly influenced the feed conversion rate (linear, P = 0.007; quadratic, P = 0.024) and the European broiler index (linear, P = 0.004; quadratic, P = 0.016) in broilers. Dietary multienzymes significantly influenced apparent metabolizable energy (quadratic, P = 0.015) and neutral detergent fiber (quadratic, P < 0.001). Moreover, multienzyme supplementation in the diet also decreased the emission of ammonia (linear, P = 0.001; quadratic, P = 0.006) and hydrogen sulfide (quadratic, P = 0.006) in the excreta. In addition, dietary multi-enzyme notably elevated (P < 0.05) the mRNA expression of nutrient transporter genes, including peptide transporter 1 (PePT1), Na-dependent neutral amino acid transporter (B0AT), glucose transporter 2 (GLUT2), and fatty acid binding protein1 (FABP1). These findings suggest that dietary supplementation with multienzymes can improve the efficiency of feed utilization, and the digestion and absorption of nutrients and reduce excreta gas emission. Furthermore, this study provides a theoretical basis for advancing the use of multienzymes in broiler production.
在禽类饲料中添加多酶制剂被认为是一种旨在改善禽类生产性能和健康状况的营养策略。然而，一些文献对多酶制剂对禽类生长性能的有益影响程度存在持续的争议。本研究旨在探讨日粮中添加多酶制剂对肉鸡的影响，重点关注生长性能、胴体特征、表观养分消化率、粪便有害气体排放和肠道养分转运体基因表达。将 3200 只肉鸡随机分为五组（每组 8 个重复），分别用以下处理：正常对照组（CON）、CON+100 g/t 多酶制剂（ME100）、CON+150 g/t 多酶制剂（ME150）、CON+200 g/t 多酶制剂（ME200）和 CON+250 g/t 多酶制剂（ME250）。添加多酶制剂显著影响肉鸡的饲料转化率（线性，P=0.007；二次，P=0.024）和欧洲肉鸡指数（线性，P=0.004；二次，P=0.016）。日粮中多酶制剂显著影响表观代谢能（二次，P=0.015）和中性洗涤纤维（二次，P<0.001）。此外，日粮中添加多酶制剂还降低了粪便中氨（线性，P=0.001；二次，P=0.006）和硫化氢（二次，P=0.006）的排放。此外，日粮多酶显著提高了养分转运体基因（包括肽转运体 1（PePT1）、Na 依赖性中性氨基酸转运体（B0AT）、葡萄糖转运体 2（GLUT2）和脂肪酸结合蛋白 1（FABP1）的 mRNA 表达（P<0.05）。这些发现表明，日粮中添加多酶制剂可以提高饲料利用率和养分的消化吸收效率，并降低粪便气体排放。此外，本研究为多酶制剂在肉鸡生产中的应用提供了理论依据。
