Messora Michel R, Pereira Luciano J, Foureaux Renata, Oliveira Luiz F F, Sordi Caio G, Alves Ana J N, Napimoga Marcelo H, Nagata Maria J H, Ervolino Edilson, Furlaneto Flávia A C
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of Sao Paulo-USP, Av. Café, 14040-904 Ribeirao Preto, SP, Brazil.
Physiology and Pharmacology Department, Federal University of Lavras, Av. Doutor Sylvio Menicucci, 1001, 37200-000 Lavras, MG, Brazil.
Arch Oral Biol. 2016 Jun;66:108-19. doi: 10.1016/j.archoralbio.2016.02.014. Epub 2016 Feb 26.
The purposes of this study were to evaluate, in rats: i) the effects of Bacillus species on the development of experimental periodontitis (EP) via microtomographic, immunological and hematological assays (Experiment 1-E1); ii) the effects of Bacillus species as adjuncts to scaling and root planing (SRP) for the treatment of EP via histomorphometric and immunohistochemical analyses (Experiment 2-E2).
In E1, 24 rats were divided into groups C1 (control), PROB1, EP1 and EP-PROB1. In groups with EP, the mandibular first molar of each animal received a ligature for 14 days. In groups PROB1, animals received Bacillus species for 44 days, starting 30 days before EP induction in Group EP-PROB1. In E2, 24 rats were assigned to groups C2 (control), PROB2, EP-SRP2 and EP-SRP-PROB2. In groups with SRP, EP was induced as described in E1. The ligatures were removed after 14 days and SRP was performed. In groups PROB2, animals received Bacillus species for 15 days, starting after SRP in Group EP-SRP-PROB2.
In E1, Group EP1 presented bone loss (BL) and eosinophil numbers greater than Group EP-PROB1 (P<0.05). In Group EP-PROB1, the receptor activator of nuclear factor-kB ligand (RANKL)/osteoprotegerin (OPG) ratio was similar to that of groups without EP. In E2, Group EP-SRP-PROB2 presented fewer TRAP-positive osteoclasts, lower immunolabeling pattern for a proinflammatory cytokine and decreased BL and attachment loss than Group EP-SRP2 (P<0.05).
Bacillus species supplementation provided a protective effect against BL and enhanced the effects of SRP in the treatment of EP in rats.
本研究旨在通过显微断层扫描、免疫学和血液学检测评估大鼠体内：i）芽孢杆菌属对实验性牙周炎（EP）发展的影响（实验1 - E1）；ii）芽孢杆菌属作为龈下刮治术和根面平整术（SRP）辅助手段治疗EP的效果，采用组织形态计量学和免疫组织化学分析（实验2 - E2）。
在E1中，将24只大鼠分为C1组（对照组）、PROB1组、EP1组和EP - PROB1组。在患有EP的组中，每只动物的下颌第一磨牙结扎14天。在PROB1组中，动物从EP - PROB1组诱导EP前30天开始接受芽孢杆菌属治疗44天。在E2中，将24只大鼠分为C2组（对照组）、PROB2组、EP - SRP2组和EP - SRP - PROB2组。在进行SRP的组中，按照E1所述诱导EP。14天后移除结扎线并进行SRP。在PROB2组中，动物从EP - SRP - PROB2组进行SRP后开始接受芽孢杆菌属治疗15天。
在E1中，EP1组的骨吸收（BL）和嗜酸性粒细胞数量高于EP - PROB1组（P<0.05）。在EP - PROB1组中，核因子 - kB受体激活剂配体（RANKL）/骨保护素（OPG）比值与无EP的组相似。在E2中，与EP - SRP2组相比，EP - SRP - PROB2组的抗酒石酸酸性磷酸酶（TRAP）阳性破骨细胞数量更少、促炎细胞因子的免疫标记模式更低，且BL和附着丧失减少（P<0.05）。
补充芽孢杆菌属对BL具有保护作用，并增强了SRP治疗大鼠EP的效果。
