Garcia Valdir Gouveia, Miessi Daniela Maria Janjácomo, Esgalha da Rocha Tiago, Gomes Natália Amanda, Nuernberg Marta Aparecida Alberton, Cardoso Jânderson de Medeiros, Ervolino Edilson, Theodoro Letícia Helena
Periodontics Division, Department of Diagnostic and Surgery, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil.
Latin American Institute of Dental Research and Education (ILAPEO), Curitiba, PR, Brazil.
Saudi Dent J. 2022 Sep;34(6):516-526. doi: 10.1016/j.sdentj.2022.05.004. Epub 2022 May 20.
The aim of this study was to evaluate the effects of probiotic (PRO) (DSM17938) as an adjuvant to the treatment of experimental periodontitis (EP).
Fifty-four male adult Wistar rats were included. EP was induced and maintained for 7 days. Subsequently, the ligature was removed and the animals were allocated into three different experimental groups (n = 18/group): EP - no local treatment, the animals received four systemic saline solution (SS) administrations; SRP+SS, the animals underwent SRP treatment, followed by SS administration; and SRP+PRO, the animals received SRP treatment, followed by the systemic administration of PROs ( ; 0.16 ml/day). Six animals from each group were euthanised at 7, 15 and 30 days. Histological and histometric analyses of alveolar bone loss (BL) and immunohistochemical analyses for TRAP, RANKL, OPG, OCN, and PCNA were performed. Shapiro-Wilk, ANOVA, post-hoc Tukey, Kruskal-Wallis, Student-Newman Keuls were performed.
The SRP+PRO group presented a reduction in inflammation. At 15 days, a lower BL was observed in the SRP+SS and SRP+PRO groups. Greater immunolabeling was noticed for PCNA at 15 days in the SRP+PRO group than in the SS group. The SRP+PRO group demonstrated a higher OCN immunolabeling pattern than the EP group at 15 and 30 days.
The use of as an adjuvant to SRP for the treatment of EP showed promising results in the control of local inflammatory responses, and enhanced the periodontal tissue repair process according to the employed concentration.
本研究旨在评估益生菌（PRO）（DSM17938）作为实验性牙周炎（EP）治疗辅助剂的效果。
纳入54只成年雄性Wistar大鼠。诱导并维持EP 7天。随后，移除结扎线，将动物分为三个不同的实验组（每组n = 18）：EP - 无局部治疗，动物接受四次全身生理盐水（SS）给药；SRP + SS，动物接受SRP治疗，随后给予SS；以及SRP + PRO，动物接受SRP治疗，随后全身给予PRO（；0.16 ml/天）。每组6只动物在第7、15和30天安乐死。进行牙槽骨吸收（BL）的组织学和组织计量学分析以及TRAP、RANKL、OPG、OCN和PCNA的免疫组织化学分析。进行Shapiro-Wilk、方差分析、事后Tukey检验、Kruskal-Wallis检验、Student-Newman Keuls检验。
SRP + PRO组炎症减轻。在第15天，SRP + SS组和SRP + PRO组观察到较低的BL。在第15天，SRP + PRO组的PCNA免疫标记比SS组更强。在第15天和30天，SRP + PRO组的OCN免疫标记模式高于EP组。
使用[具体益生菌名称未给出]作为SRP治疗EP的辅助剂在控制局部炎症反应方面显示出有前景的结果，并根据所用浓度增强了牙周组织修复过程。
