Lan Jingwen, Chen Jinyang, Li Ningxing, Ji Xinghu, Yu Mingxia, He Zhike
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Department of Clinical Laboratory & Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China.
Talanta. 2016 May 1;151:126-131. doi: 10.1016/j.talanta.2016.01.024. Epub 2016 Jan 13.
Fluorescent magnetic multifunctional microparticles were fabricated by a facile droplet microfluidic strategy. Two sodium alginate streams, one doped with Fe3O4 nanoparticles (NPs) and the other with CdSe/ZnS quantum dots, were introduced into a flow-focusing channel as a type of parallel laminar flow to form droplets containing two distinct parts. Then, at the serpentine channel, the Ca(2+) in the oil phase diffused into the droplets, causing the solidification of the droplets. Thus, the Janus microparticles with excellent magnetic/fluorescent properties formed. The flow conditions were optimized and the effects of the flow rates on magnetic/fluorescent compositions were carefully investigated. Luminescent labeling and magnetic separation were simultaneously realized with the newly designed microparticles. Moreover, spatial separation between Fe3O4 NPs and QDs prevented the interference of QDs photoluminescence by the magnetic particles. The as-prepared fluorescent magnetic Janus particles were also successfully employed for DNA assay, which demonstrated the potential of the multifunctional microbeads in biological applications.
通过一种简便的微滴微流控策略制备了荧光磁性多功能微粒。将两股海藻酸钠流，一股掺杂有Fe3O4纳米颗粒（NPs），另一股掺杂有CdSe/ZnS量子点，作为一种平行层流引入到流动聚焦通道中，以形成包含两个不同部分的液滴。然后，在蜿蜒通道中，油相中的Ca(2+)扩散到液滴中，导致液滴固化。由此，形成了具有优异磁/荧光性能的Janus微粒。优化了流动条件，并仔细研究了流速对磁/荧光组成的影响。新设计的微粒同时实现了发光标记和磁分离。此外，Fe3O4 NPs和量子点之间的空间分离防止了磁性颗粒对量子点光致发光的干扰。所制备的荧光磁性Janus颗粒也成功用于DNA检测，这证明了多功能微珠在生物应用中的潜力。
