Alnawaiseh Maged, Rosentreter André, Hillmann Anja, Alex Anne F, Niekämper Daniel, Heiduschka Peter, Pap Thomas, Eter Nicole
Dept. of Ophthalmology, University of Muenster Medical Center, Muenster, Germany; Institute of Experimental Musculoskeletal Medicine, University of Muenster Medical Center, Muenster, Germany.
Dept. of Ophthalmology, University of Muenster Medical Center, Muenster, Germany; Dept. of Ophthalmology, University of Würzburg, Würzburg, Germany.
Exp Eye Res. 2016 Apr;145:417-423. doi: 10.1016/j.exer.2016.02.012. Epub 2016 Mar 2.
To investigate the application of optical coherence tomography (OCT) angiography in the retinas of healthy mice and to evaluate choroidal neovascularization (CNV) in a mouse model of laser-induced CNV.
C57BL/6J mice aged 18-25 weeks were examined using the spectral-domain optical coherence tomography device RTVue XR Avanti (Optovue, Inc, Fremont, California, USA). Blood flow in different retinal layers was detected using the split-spectrum amplitude-decorrelation angiography algorithm. Fluorescein angiography (FA) images were obtained using the Heidelberg Spectralis device (Heidelberg, Germany).
Using the RTVue XR Avanti, we were able to obtain high-quality OCT angiography images of normal vasculature in the superficial, deep capillary and choriocapillary layers in laser-treated mice and untreated controls. Whereas no blood flow was detectable in the outer retina of untreated mice, blood flow and hence neovascular vessels were found in laser-treated mice.
OCT angiography can clearly visualize the normal vascular plexus in the different retinal layers in the mouse retina and choroid. With OCT angiography, it is possible to verify the choroidal neovascularization induced by laser treatment. Thus, OCT angiography is a helpful imaging tool for non-invasive, in vivo evaluation of laser-induced CNV in the mouse.
研究光学相干断层扫描（OCT）血管造影术在健康小鼠视网膜中的应用，并评估激光诱导脉络膜新生血管（CNV）小鼠模型中的脉络膜新生血管情况。
使用光谱域光学相干断层扫描设备RTVue XR Avanti（美国加利福尼亚州弗里蒙特市Optovue公司）对18 - 25周龄的C57BL/6J小鼠进行检查。采用分谱振幅去相关血管造影算法检测不同视网膜层的血流情况。使用德国海德堡的Spectralis设备获取荧光素血管造影（FA）图像。
使用RTVue XR Avanti，我们能够在激光治疗小鼠和未治疗对照小鼠中获得浅层、深层毛细血管层和脉络膜毛细血管层正常血管系统的高质量OCT血管造影图像。未治疗小鼠的视网膜外层未检测到血流，而激光治疗小鼠中发现了血流及新生血管。
OCT血管造影术能够清晰显示小鼠视网膜和脉络膜不同视网膜层中的正常血管丛。通过OCT血管造影术，可以验证激光治疗诱导的脉络膜新生血管。因此，OCT血管造影术是一种有助于对小鼠激光诱导的CNV进行非侵入性体内评估的成像工具。
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