Schmidt H, Naumann G
Z Gesamte Hyg. 1989 Oct;35(10):585-8.
According to our experience in identifying coagulase negative staphylococci (CNS) a modified Kloos-Schleifer-scheme proved useful in species identification. Of 595 urine isolates from cases with significant bacteriuria tested 89.5% could be identified. For routine identification on the one hand the Kloos-Schleifer-scheme is only practical by using commercial identification systems, on the other hand applying an abbreviated scheme species identification is unsatisfactory. As an alternative for identification of CNS in clinical microbiology the Phosphatase-Novobiocin-Mannose-Inhibition-Test (PNMI-Test) is presented. This test in combination with a coagulase test proved suitable to identify the three most important staphylococcal species S. aureus, S epidermidis, and S. saprophyticus and seems to be an acceptable compromise in the bacteriological routine laboratory. For extended species identification of CNS the Kloos-Schleifer-scheme should not be reduced but this identification should be reserved to special laboratories.
根据我们鉴定凝固酶阴性葡萄球菌（CNS）的经验，改良的克洛斯-施莱弗分类法在菌种鉴定中被证明是有用的。在对595例有显著菌尿症病例的尿液分离株进行检测后，89.5%的菌株能够被鉴定出来。一方面，对于常规鉴定而言，克洛斯-施莱弗分类法只有通过使用商业鉴定系统才切实可行；另一方面，应用简化分类法进行菌种鉴定则不尽人意。作为临床微生物学中鉴定CNS的替代方法，本文介绍了磷酸酶-新生霉素-甘露糖抑制试验（PNMI试验）。该试验与凝固酶试验相结合，被证明适用于鉴定三种最重要的葡萄球菌菌种，即金黄色葡萄球菌、表皮葡萄球菌和腐生葡萄球菌，并且在细菌学常规实验室中似乎是一个可以接受的折衷方法。对于CNS的扩展菌种鉴定，不应简化克洛斯-施莱弗分类法，而这种鉴定应保留给专业实验室。
