BAE: Biocapteurs-Analyss-Environnement, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France; Polymeric Materials Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, P.O. Box: 21934, Egypt.
Polymeric Materials Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, P.O. Box: 21934, Egypt.
Food Chem. 2016 Jul 15;203:73-78. doi: 10.1016/j.foodchem.2016.02.014. Epub 2016 Feb 2.
An ultra-sensitive screen-printed biosensor was successfully developed for phosmet detection in olive oil, based on a genetically-engineered acetylcholinesterase (AChE) immobilized in a azide-unit water-pendant polyvinyl alcohol (PVA-AWP)/Fe-Ni alloy nanocomposite. Fe-Ni not only allowed amplifying the response current but also lowering the applied potential from 80 mV to 30 mV vs Ag/AgCl. The biosensor showed a very good analytical performance for phosmet detection, with a detection limit of 0.1 nM. This detection limit is lower than the allowable concentrations set by international regulations. In addition to the good reproducibility, operational and storage stability, the developed biosensor was successfully used for the determination of phosmet in olive oil samples without any laborious pre-treatment. The phosmet recovery rate was about 96% after a simple liquid-liquid extraction.
一种超灵敏的丝网印刷生物传感器成功地被开发出来，用于橄榄油中 phosmet 的检测，其基于固定在叠氮单元水悬聚 乙烯醇（PVA-AWP）/Fe-Ni 合金纳米复合材料中的基因工程乙酰胆碱酯酶（AChE）。Fe-Ni 不仅允许放大响应电流，还将施加的电势从 80 mV 降低到 30 mV 相对于 Ag/AgCl。该生物传感器对 phosmet 的检测表现出非常好的分析性能，检测限为 0.1 nM。这个检测限低于国际法规规定的允许浓度。除了良好的重现性、操作稳定性和储存稳定性外，开发的生物传感器还成功地用于橄榄油样品中 phosmet 的测定，无需进行繁琐的预处理。经过简单的液-液萃取，phosmet 的回收率约为 96%。
