El-Moghazy A Y, Soliman E A, Ibrahim H Z, Marty J-L, Istamboulie G, Noguer T
Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, Egypt.
Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria 832, Egypt.
Talanta. 2016 Aug 1;155:258-64. doi: 10.1016/j.talanta.2016.04.018. Epub 2016 Apr 22.
An ultra-sensitive electrochemical biosensor was successfully developed for rapid detection of pirimiphos-methyl in olive oil, based of genetically-engineered acetylcholinesterase (AChE) immobilization into electrospun chitosan/poly (vinyl alcohol) blend nanofibers. Due to their unique properties such as spatial structure, high porosity, and large surface area, the use of nanofibers allowed improving the biosensor response by two folds. The developed biosensor showed a good performance for detecting pirimiphos-methyl, with a limit of detection of 0.2nM, a concentration much lower than the maximum residue limit allowed set by international regulations (164nM). The biosensor was used for the detection of pirimiphos-methyl in olive oil samples after a simple liquid-liquid extraction, and the recovery rates were close to 100%.
基于将基因工程乙酰胆碱酯酶（AChE）固定在电纺壳聚糖/聚乙烯醇混合纳米纤维中，成功开发了一种超灵敏电化学生物传感器，用于快速检测橄榄油中的甲基嘧啶磷。由于纳米纤维具有独特的性质，如空间结构、高孔隙率和大表面积，使用纳米纤维可使生物传感器的响应提高两倍。所开发的生物传感器在检测甲基嘧啶磷方面表现良好，检测限为0.2nM，该浓度远低于国际法规规定的最大残留限量（164nM）。该生物传感器经简单液液萃取后用于检测橄榄油样品中的甲基嘧啶磷，回收率接近100%。
