Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore 54000, Pakistan; Interdisciplinary Research Center in Biomedical Materials (IRCBM), COMSATS University, Islamabad, Lahore Campus, Lahore 54000, Pakistan.
Interdisciplinary Research Center in Biomedical Materials (IRCBM), COMSATS University, Islamabad, Lahore Campus, Lahore 54000, Pakistan.
Food Chem. 2021 Jun 1;346:128894. doi: 10.1016/j.foodchem.2020.128894. Epub 2021 Jan 7.
This study was undertaken to assess the potential of Tribolium castaneum (Red flour beetle) acetylcholinesterase (Tc-AChE) based electrochemical biosensor integrating WO/g-CN nanocomposite modified Pencil graphite electrode to detect an organophosphate insecticide, Phosmet. The WO/g-CN nanocomposite provides a non-toxic, biocompatible surface for binding the enzyme on the electrode surface, attributed to its large surface area, high conductivity, and low ohmic resistance. The proposed biosensor shows a very good analytical performance with LOD 3.6 nM for Phosmet and effectively determined Phosmet in wheat with a 99% recovery rate. Furthermore, molecular docking deciphers the binding interactions of Phosmet with Tc-AChE using a modified AutoDock LGA algorithm and an AMBER03 force field in YASARA. The kinetic parameters strongly suggest the high potency of inhibitor with the enzyme. This study presents an adaptable, rapid, and straightforward approach that opens ways towards real progress in developing commercial biosensors for pesticide detection.
本研究旨在评估基于赤拟谷盗乙酰胆碱酯酶（Tc-AChE）的电化学生物传感器的潜力，该传感器集成了 WO/g-CN 纳米复合材料修饰的铅笔石墨电极，用于检测有机磷杀虫剂灭线磷。WO/g-CN 纳米复合材料为酶在电极表面的结合提供了无毒、生物相容的表面，这归因于其大的表面积、高导电性和低欧姆电阻。所提出的生物传感器对灭线磷具有非常好的分析性能，LOD 为 3.6 nM，并有效地用 99%的回收率测定了小麦中的灭线磷。此外，分子对接使用经过修改的 AutoDock LGA 算法和 YASARA 中的 AMBER03 力场来破译灭线磷与 Tc-AChE 的结合相互作用。动力学参数强烈表明抑制剂与酶具有高效力。本研究提出了一种适应性强、快速且简单的方法，为开发用于农药检测的商业生物传感器开辟了新的途径。
