Yassin Sufian A, German Matthew J, Rolland Sarah L, Rickard Alexander H, Jakubovics Nicholas S
School of Dental Sciences, Centre for Oral Health Research, Newcastle University, Newcastle upon Tyne NE2 4BW, UK.
Department of Epidemiology, University of Michigan, Ann Arbor, MI 48109-2029, USA.
J Dent. 2016 May;48:62-70. doi: 10.1016/j.jdent.2016.03.001. Epub 2016 Mar 4.
This study aimed to develop a new mixed-species acidogenic biofilm model and use it to assess the antimicrobial properties of a novel fluoride-releasing copolymer.
Stubs composed of a copolymer of methyl methacrylate (MMA) and 2-hydroxyethyl methacrylate (HEMA) with polymethyl methacrylate (PMMA) were produced by chemically-activated free radical polymerization. A fluoride-releasing copolymer was developed by incorporating sodium fluoride in place of a portion of the PMMA. Samples were mounted in polysulfone Modified Robbins Devices (MRDs) and were optimized for single- and mixed-species biofilm formation by Candida albicans, Lactobacillus casei and Streptococcus mutans.
Fluoride release was sustained for at least 48h in flowing conditions. Fluoride did not affect the colonization and biofilm growth of any of the microorganisms in monocultures. However, in mixed-species biofilms, cell densities of all three species were reduced approximately ten-fold (p<0.05) on the fluoridated material compared with the non-fluoridated copolymer.
These data demonstrate that intermicrobial interactions in mixed-species acidogenic biofilms are sensitive to fluoride, and that the inclusion of fluoride in a denture lining copolymer reduces the formation of polymicrobial biofilms.
The growth of acidogenic microorganisms on denture materials is associated with denture stomatitis and dental caries on surrounding teeth. A fluoride-releasing copolymer that inhibits acidogenic mixed-species biofilms, such as the material described in this study, has the potential to control these diseases by limiting biofilm growth.
本研究旨在开发一种新的混合菌种产酸生物膜模型，并使用该模型评估一种新型含氟共聚物的抗菌性能。
通过化学活化自由基聚合反应制备由甲基丙烯酸甲酯（MMA）和甲基丙烯酸2-羟乙酯（HEMA）与聚甲基丙烯酸甲酯（PMMA）组成的共聚物短棒。通过用氟化钠取代部分PMMA来开发一种含氟共聚物。将样品安装在聚砜改良罗宾斯装置（MRD）中，并针对白色念珠菌、干酪乳杆菌和变形链球菌的单菌种和混合菌种生物膜形成进行优化。
在流动条件下，氟化物释放持续至少48小时。氟化物不影响单菌种培养中任何微生物的定植和生物膜生长。然而，在混合菌种生物膜中，与未氟化的共聚物相比，氟化材料上所有三种菌种的细胞密度降低了约10倍（p<0.05）。
这些数据表明，混合菌种产酸生物膜中的微生物间相互作用对氟化物敏感，并且在义齿衬里共聚物中加入氟化物可减少多菌种生物膜的形成。
产酸微生物在义齿材料上的生长与义齿性口炎和周围牙齿的龋齿有关。一种抑制产酸混合菌种生物膜的含氟共聚物，如本研究中所述的材料，有可能通过限制生物膜生长来控制这些疾病。
