Zhang Keke, Ren Biao, Zhou Xuedong, Xu Hockin H K, Chen Yu, Han Qi, Li Bolei, Weir Michael D, Li Mingyun, Feng Mingye, Cheng Lei
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Int J Mol Sci. 2016 Jun 29;17(7):1033. doi: 10.3390/ijms17071033.
Our aims of the research were to study the antimicrobial effect of dimethylaminododecyl methacrylate (DMADDM) modified denture base resin on multi-species biofilms and the biocompatibility of this modified dental material. Candida albicans (C. albicans), Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis), as well as Actinomyces naeslundii (A. naeslundii) were used for biofilm formation on denture base resin. Colony forming unit (CFU) counts, microbial viability staining, and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) array were used to evaluate the antimicrobial effect of DMADDM. C. albicans staining and Real-time PCR were used to analyze the morphology and expression of virulence genes of C. albicans in biofilm. Lactate dehydrogenase (LDH) array and Real-time PCR were conducted to examine the results after biofilm co-cultured with epithelial cell. Hematoxylin and eosin (HE) staining followed by histological evaluation were used to study the biocompatibility of this modified material. We found that DMADDM containing groups reduced both biomass and metabolic activity of the biofilm significantly. DMADDM can also inhibit the virulence of C. albicans by means of inhibiting the hyphal development and downregulation of two virulence related genes. DMADDM significantly reduced the cell damage caused by multi-species biofilm according to the LDH activity and reduced the expression of IL-18 gene of the cells simultaneously. The in vivo histological evaluation proved that the addition of DMADDM less than 6.6% in denture material did not increase the inflammatory response (p > 0.05). Therefore, we proposed that the novel denture base resin containing DMADDM may be considered as a new promising therapeutic system against problems caused by microbes on denture base such as denture stomatitis.
我们的研究目的是研究甲基丙烯酸二甲氨基十二酯（DMADDM）改性义齿基托树脂对多物种生物膜的抗菌作用以及这种改性牙科材料的生物相容性。白色念珠菌（白色念珠菌）、变形链球菌（变形链球菌）、血链球菌（血链球菌）以及内氏放线菌（内氏放线菌）用于在义齿基托树脂上形成生物膜。采用菌落形成单位（CFU）计数、微生物活力染色和2,3-双（2-甲氧基-4-硝基-5-磺基苯基）-2H-四唑-5-羧苯胺（XTT）阵列评估DMADDM的抗菌效果。白色念珠菌染色和实时聚合酶链反应用于分析生物膜中白色念珠菌的形态和毒力基因表达。进行乳酸脱氢酶（LDH）阵列和实时聚合酶链反应以检测生物膜与上皮细胞共培养后的结果。苏木精和伊红（HE）染色后进行组织学评估以研究这种改性材料的生物相容性。我们发现含DMADDM的组显著降低了生物膜的生物量和代谢活性。DMADDM还可通过抑制菌丝发育和下调两个毒力相关基因来抑制白色念珠菌的毒力。根据LDH活性，DMADDM显著降低了多物种生物膜引起的细胞损伤，同时降低了细胞中IL-18基因的表达。体内组织学评估证明，义齿材料中DMADDM添加量小于6.6%不会增加炎症反应（p>0.05）。因此，我们提出含DMADDM的新型义齿基托树脂可被视为一种有前景的新型治疗系统来解决义齿基托上微生物引起的问题，如义齿性口炎。
