CI&DEST, Escola Superior de Tecnologia e Gestão de Viseu, Instituto Politécnico de Viseu, 3504-510 Viseu, Portugal; LEPABE, Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; LEAF, Centro de Investigação em Agronomia, Alimentos, Ambiente e Paisagem, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
LEPABE, Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
Waste Manag. 2016 Apr;50:20-30. doi: 10.1016/j.wasman.2016.02.023. Epub 2016 Mar 2.
Previously, two municipal solid waste commercial composts (MSW1 and MSW2) were characterized. Although sharing the same type of raw material, most of their physicochemical, stability and maturity properties differed. The present study aimed to characterize them at a microbiological level, and to infer on possible relationships between the composts properties and the structure of their bacterial communities. Both the 16S rRNA gene-based PCR-DGGE profiling and 454-pyrosequencing technology showed that the structure of the bacterial communities of these composts was distinct. The bacterial community of MSW1 was more diverse than that of MSW2. Multivariate analyses revealed that the high electrical conductivity, Cu content as well as the low phytotoxity of compost MSW1, when compared to MSW2, contributed most to shape its bacterial community structure. Indeed, high abundance of halophilic (Halomonadaceae and Brevibacteriaceae) and metal resistant organisms (Brevibacteriaceae and Bacillaceae) were found in MSW1. In addition, Pseudonocardiaceae, Streptomycetaceae, Bacillaceae, and Brevibacteriaceae may have contributed to the high humic-like acids content and low phytotoxicity of MSW1. In contrast, the high organic matter content and the high density of the cultivable fungi population were the parameters most correlated with the structure of the bacterial community of compost MSW2, dominated by Corynebacteriaceae and mainly Aerococcaceae, taxonomic groups not commonly found in composts.
先前对两份商业厨余堆肥（MSW1 和 MSW2）进行了特性描述。尽管它们的原料相同，但大部分理化性质、稳定性和成熟度参数存在差异。本研究旨在从微生物学水平对它们进行特征描述，并推断堆肥特性与细菌群落结构之间可能存在的关系。基于 16S rRNA 基因的 PCR-DGGE 指纹图谱和 454 焦磷酸测序技术均表明，这两种堆肥的细菌群落结构截然不同。MSW1 的细菌群落多样性比 MSW2 更丰富。多元分析表明，与 MSW2 相比，MSW1 具有较高的电导率、Cu 含量和较低的植物毒性，这是导致其细菌群落结构形成的主要原因。实际上，在 MSW1 中发现了丰富的嗜盐菌（盐单胞菌科和短杆菌科）和耐金属菌（短杆菌科和芽孢杆菌科）。此外，诺卡氏菌科、链霉菌科、芽孢杆菌科和短杆菌科可能有助于 MSW1 中腐殖酸含量高和植物毒性低。相比之下，高有机物含量和可培养真菌种群密度是与 MSW2 细菌群落结构最相关的参数，其细菌群落由棒状杆菌科和主要的微球菌科主导，这些分类群在堆肥中并不常见。
