Munk Michele, Camargo Luiz S A, Quintão Carolina C R, Silva Saulo R, Souza Eliza D, Raposo Nádia R B, Marconcini Jose M, Jorio Ado, Ladeira Luiz O, Brandão Humberto M
Department of Biology, Federal University of Juiz de Fora, Juiz de Fora, Brazil.
Embrapa Dairy Cattle (CNPGL), Juiz de Fora, Brazil.
Nanomedicine. 2016 Jul;12(5):1151-9. doi: 10.1016/j.nano.2016.01.006. Epub 2016 Mar 3.
Currently there is a growing interest in the use of nanotechnology in reproductive medicine and reproductive biology. However, their toxic effects on mammalian embryos remain poorly understood. In this work, we evaluate the biocompatibility of two fibrous nanomaterials (NMs): cotton cellulose nanofibers (CNF) and carboxylated multiwalled carbon nanotubes (MWCNT-COOH), by performing an investigation of the embryonic development, gene expression (biomarkers focused on cell stress, apoptosis and totipotency) and in situ apoptosis in bovine embryos. Exposure to NMs did not interfere in preimplantation development or in the incidence of apoptosis in the bovine embryo, but they did affect the gene expression. The results presented are important for an understanding of the toxicity of cotton CNF and MWCNT-COOH on mammalian embryos. To our knowledge, we report the first evaluation of biocompatibility between these NMs on preimplantation embryos, which may open a new window for reproductive biomedical applications.
目前，纳米技术在生殖医学和生殖生物学中的应用越来越受到关注。然而，它们对哺乳动物胚胎的毒性作用仍知之甚少。在这项工作中，我们通过对牛胚胎的胚胎发育、基因表达（关注细胞应激、凋亡和全能性的生物标志物）以及原位凋亡进行研究，评估了两种纤维状纳米材料（NMs）的生物相容性：棉纤维素纳米纤维（CNF）和羧基化多壁碳纳米管（MWCNT-COOH）。暴露于纳米材料不会干扰牛胚胎的植入前发育或凋亡发生率，但会影响基因表达。所呈现的结果对于理解棉CNF和MWCNT-COOH对哺乳动物胚胎的毒性很重要。据我们所知，我们首次报道了这些纳米材料对植入前胚胎生物相容性的评估，这可能为生殖生物医学应用打开一扇新窗口。
