Department of Science Education, National Taipei University of Education, No.134, Sec. 2, Heping E. Rd., Da-an District, Taipei City 106, Taiwan.
School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wuxing Street, Taipei City 110, Taiwan.
J Biotechnol. 2019 Apr 20;296:14-21. doi: 10.1016/j.jbiotec.2019.02.005. Epub 2019 Mar 7.
Carbon nanomaterials, include carbon nanotubes and graphene nanosheets, have drawn an increasing amount of attention because of their potential applications in daily life or in providing novel therapeutic possibilities for treating diseases. However, the overall biocompatibility, the potential toxic effects of carbon nanomaterials toward human cells, and their modulations in cellular mechanism, are not fully understood. Herein, four types of carbon nanomaterials, include long and short carbon nanotubes and graphene nanosheets, at low and high concentrations, were functionalized and dispersed in the biocompatible buffer for assessment. The surface structure, the morphology, and chemical composition of carbon nanomaterials were characterized. Also, biological assays investigating cellular viability, vitality, cell cycle, and apoptotic cell death were applied on cells co-incubated with nanomaterials, to evaluate the biocompatibility of these nanomaterials in human cells. Our data suggested that even though co-incubation of nanomaterials did not seem to affect the viability of cells notably, high concentrations (50 ug/ml) of SW could lead to unhealthy cells, and we observed dramatic G2 arrest effect mediated by p21 induction in high SW incubated cells. Other nanomaterials at high concentration may also alter cell cycle profile of the cells. In summary, our data demonstrated that these nanomaterials could regulate cell cycle and lead to apoptosis at high concentrations, and the underling molecular mechanisms have been addressed. Caution should be taken on their concentration when nanomaterials are in used in future medical applications.
碳纳米材料，包括碳纳米管和石墨烯纳米片，由于其在日常生活中的潜在应用或为治疗疾病提供新的治疗可能性，引起了越来越多的关注。然而，碳纳米材料的整体生物相容性、对人类细胞的潜在毒性作用及其对细胞机制的调节作用尚不完全清楚。在此，我们将四种类型的碳纳米材料，包括长和短的碳纳米管和石墨烯纳米片，在低浓度和高浓度下进行功能化和分散在生物相容性缓冲液中进行评估。我们对碳纳米材料的表面结构、形态和化学组成进行了表征。此外，我们还应用了细胞活力、活力、细胞周期和凋亡细胞死亡的生物测定法来研究与纳米材料共孵育的细胞，以评估这些纳米材料在人类细胞中的生物相容性。我们的数据表明，尽管纳米材料共孵育似乎不会显著影响细胞的活力，但高浓度（50ug/ml）的 SW 可能导致细胞不健康，我们观察到高浓度 SW 孵育的细胞中 p21 诱导导致明显的 G2 期阻滞效应。其他纳米材料在高浓度时也可能改变细胞的细胞周期谱。总之，我们的数据表明，这些纳米材料可以在高浓度时调节细胞周期并导致细胞凋亡，并探讨了潜在的分子机制。在未来的医学应用中，应注意纳米材料的浓度。
