Zhang Xiaoli, Wang Litao, Dong Shuqing, Zhang Xia, Wu Qi, Zhao Liang, Shi Yanping
Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Science, Lanzhou, Gansu Province, P.R. China.
University of Chinese Academy of Sciences, Beijing, P.R. China.
Chirality. 2016 May;28(5):376-81. doi: 10.1002/chir.22578. Epub 2016 Mar 6.
Nanocrystalline cellulose (NCC) with high surface area and high ordered crystalline structure was prepared from microcrystalline cellulose (MCC) under the hydrolysis of sodium hypochlorite. NCC was further reacted with 3,5-dimethylphenyl isocyanate to obtain the nanocellulose derivative, and then coated successfully on the surface of silica gel to a prepared NCC-coated chiral stationary phase (CSP) as a new kind of chiral separation material. Similarly, MCC derivative-coated CSP was also prepared as contrast. The chiral separation performance of NCC-based CSP was evaluated and compared with MCC-based CSP by high-performance liquid chromatography. Moreover, the effects of the alcohol modifiers, mobile phase additives, and flow rates on chiral separations were investigated in detail. The results showed that 10 chiral compounds were separated on NCC-based CSP with better peak shape and higher column efficiency than MCC-based CSP, which confirmed that NCC-based CSP was a promising packing material for the resolution of chiral compounds.Chirality 28:376-381, 2016. © 2016 Wiley Periodicals, Inc.
在次氯酸钠水解作用下，由微晶纤维素（MCC）制备出具有高比表面积和高有序晶体结构的纳米晶纤维素（NCC）。NCC进一步与3,5-二甲基苯基异氰酸酯反应，得到纳米纤维素衍生物，然后成功地包覆在硅胶表面，制备出一种新型手性分离材料——NCC包覆的手性固定相（CSP）。同样，制备了MCC衍生物包覆的CSP作为对照。通过高效液相色谱法评估了基于NCC的CSP的手性分离性能，并与基于MCC的CSP进行了比较。此外，还详细研究了醇类改性剂、流动相添加剂和流速对手性分离的影响。结果表明，在基于NCC的CSP上分离出了10种手性化合物，其峰形比基于MCC的CSP更好，柱效更高，这证实了基于NCC的CSP是一种有前途的用于拆分手性化合物的填充材料。《手性》28:376 - 381, 2016。© 2016威利期刊公司。
