月见草（Oenothera gayleana）和柳叶月见草（O. hartwegii subsp. filifolia）（柳叶菜科）的微卫星标记及其在萼花月见草组中的应用。
Microsatellites for Oenothera gayleana and O. hartwegii subsp. filifolia (Onagraceae), and their utility in section Calylophus.
作者信息
Lewis Emily M, Fant Jeremie B, Moore Michael J, Hastings Amy P, Larson Erica L, Agrawal Anurag A, Skogen Krissa A
机构信息
Plant Biology and Conservation, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA; Program in Plant Biology and Conservation, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208 USA.
Plant Biology and Conservation, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA.
出版信息
Appl Plant Sci. 2016 Feb 9;4(2). doi: 10.3732/apps.1500107. eCollection 2016 Feb.
PREMISE OF THE STUDY
Eleven nuclear and four plastid microsatellite markers were screened for two gypsum endemic species, Oenothera gayleana and O. hartwegii subsp. filifolia, and tested for cross-amplification in the remaining 11 taxa within Oenothera sect. Calylophus (Onagraceae).
METHODS AND RESULTS
Microsatellite markers were tested in two to three populations spanning the ranges of both O. gayleana and O. hartwegii subsp. filifolia. The nuclear microsatellite loci consisted of both di- and trinucleotide repeats with one to 17 alleles per population. Several loci showed significant deviation from Hardy-Weinberg equilibrium, which may be evidence of chromosomal rings. The plastid microsatellite markers identified one to seven haplotypes per population. The transferability of these markers was confirmed in all 11 taxa within Oenothera sect. Calylophus.
CONCLUSIONS
The microsatellite loci characterized here are the first developed and tested in Oenothera sect. Calylophus. These markers will be used to assess whether pollinator foraging distance influences population genetic parameters in predictable ways.
研究前提
针对两种石膏特有物种，即盖氏月见草（Oenothera gayleana）和丝状叶哈特维希月见草亚种（O. hartwegii subsp. filifolia），筛选了11个核微卫星标记和4个质体微卫星标记，并在月见草属萼花组（柳叶菜科）的其余11个分类群中测试了交叉扩增情况。
方法与结果
在跨越盖氏月见草和丝状叶哈特维希月见草亚种分布范围的两到三个种群中测试了微卫星标记。核微卫星位点由二核苷酸和三核苷酸重复序列组成，每个种群有1至17个等位基因。几个位点显示出显著偏离哈迪 - 温伯格平衡，这可能是染色体环的证据。质体微卫星标记在每个种群中鉴定出1至7个单倍型。这些标记在月见草属萼花组的所有11个分类群中均证实具有可转移性。
结论
此处表征的微卫星位点是首次在月见草属萼花组中开发和测试的。这些标记将用于评估传粉者觅食距离是否以可预测的方式影响种群遗传参数。
Zotero 插件