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Stratified analysis of the soil seed bank in the cedar glade endemic Astragalus bibullatus: evidence for historical changes in genetic structure.对雪松沼地特有植物双泡黄耆土壤种子库的分层分析：遗传结构历史变化的证据
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Applications of next generation sequencing in molecular ecology of non-model organisms.下一代测序在非模式生物分子生态学中的应用。
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Population genetics of Astragalus bibullatus (Fabaceae) using AFLPs.利用扩增片段长度多态性（AFLP）对双泡黄耆（豆科）进行群体遗传学研究。
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Microsatellites for ecologists: a practical guide to using and evaluating microsatellite markers.面向生态学家的微卫星：使用和评估微卫星标记的实用指南。
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联邦濒危植物双泡黄耆（豆科）微卫星引物的开发与特性分析

Development and characterization of microsatellite primers in the federally endangered Astragalus bibullatus (Fabaceae).

作者信息

Morris Ashley B, Scalf Cassandra, Burleyson Austin, Johnson La Tonya, Trostel Kevin

机构信息

Department of Biology, Middle Tennessee State University, 1301 E. Main Street, Murfreesboro, Tennessee 37132 USA.

出版信息

Appl Plant Sci. 2016 Apr 5;4(4). doi: 10.3732/apps.1500126. eCollection 2016 Apr.

DOI:10.3732/apps.1500126
PMID:27144107
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4850055/
Abstract

PREMISE OF THE STUDY

Microsatellites were developed for Astragalus bibullatus (Fabaceae), a federally endangered narrow endemic, to investigate reproductive ecology and species boundaries among closely related taxa.

METHODS AND RESULTS

Next-generation sequencing was used to develop 12 nuclear microsatellite loci that amplify in A. bibullatus, as well as in A. crassicarpus var. trichocalyx, A. gypsodes, and A. tennesseensis. Identified loci were di- and trinucleotide repeats, with 1-15 alleles per locus. Observed and expected heterozygosities ranged from 0.000-0.938 and 0.000-0.860, respectively. Cross-amplification of three loci previously published in A. michauxii was also confirmed for the taxa included here.

CONCLUSIONS

These data indicate the utility of novel microsatellite loci for conservation genetics and reproductive ecology in closely related Astragalus species.

摘要

研究前提

为豆科黄芪属的联邦濒危狭域特有种双泡黄芪开发微卫星标记，以研究其生殖生态学以及近缘类群间的物种界限。

方法与结果

利用二代测序技术开发了12个在双泡黄芪以及粗果黄芪毛萼变种、石膏黄芪和田纳西黄芪中均能扩增的核微卫星位点。鉴定出的位点为二核苷酸和三核苷酸重复序列，每个位点有1至15个等位基因。观察杂合度和期望杂合度分别为0.000至0.938和0.000至0.860。这里所包含的类群对先前在米氏黄芪中发表的3个位点的交叉扩增也得到了证实。

结论

这些数据表明了新型微卫星位点在近缘黄芪属物种的保护遗传学和生殖生态学研究中的实用性。