Nelson M J, Montgomery S O, Pritchard P H
Technology Applications, Inc., Sabine Island, Gulf Breeze, Florida.
Appl Environ Microbiol. 1988 Feb;54(2):604-6. doi: 10.1128/aem.54.2.604-606.1988.
Trichloroethylene (TCE) was metabolized by the natural microflora of three different environmental water samples when stimulated by the addition of either toluene or phenol. Two different strains of Pseudomonas putida that degrade toluene by a pathway containing a toluene dioxygenase also metabolized TCE. A mutant of one of these strains lacking an active toluene dioxygenase could not degrade TCE, but spontaneous revertants for toluene degradation also regained TCE-degradative ability. The results implicate toluene dioxygenase in TCE metabolism.
当添加甲苯或苯酚进行刺激时,三氯乙烯(TCE)可被三种不同环境水样中的天然微生物群落代谢。两种通过含有甲苯双加氧酶的途径降解甲苯的恶臭假单胞菌菌株也能代谢三氯乙烯。其中一种缺乏活性甲苯双加氧酶的菌株突变体无法降解三氯乙烯,但甲苯降解的自发回复突变体也恢复了三氯乙烯降解能力。结果表明甲苯双加氧酶参与了三氯乙烯的代谢。