Neves Ana Rute, Martins Susana, Segundo Marcela A, Reis Salette
UCIBIO, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
Laboratory for Pharmaceutical Technology/Research Centre in Pharmaceutical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
Nutrients. 2016 Mar 2;8(3):131. doi: 10.3390/nu8030131.
Resveratrol was investigated in terms of its stability, biocompatibility and intestinal permeability across Caco-2 cell monolayers in its free form or encapsulated in solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs). SLNs and NLCs presented a mean diameter between 160 and 190 nm, high negative zeta potential of -30 mV and resveratrol entrapment efficiency of 80%, suggesting they are suitable for resveratrol oral delivery. Nanoencapsulation effectively protected resveratrol from photodegradation, and MTT assays demonstrated that neither resveratrol nor lipid nanoparticles adversely affected cell viability and integrity of Caco-2 cell monolayers. The in vitro intestinal permeability of resveratrol was significantly increased by NLCs, and SLNs did not impair the absorption of resveratrol. Resveratrol oral absorption can be enhanced during meals, since the intestinal permeability was increased in the presence of fed-state intestinal juices. SLNs and NLCs constitute carrier systems for resveratrol oral administration, for further use as supplements or nutraceuticals.
研究了白藜芦醇以游离形式或包封于固体脂质纳米粒（SLNs）和纳米结构脂质载体（NLCs）中的稳定性、生物相容性及其跨Caco-2细胞单层的肠道通透性。SLNs和NLCs的平均直径在160至190nm之间，具有-30mV的高负zeta电位和80%的白藜芦醇包封率，表明它们适用于白藜芦醇的口服递送。纳米包封有效地保护白藜芦醇免受光降解，MTT试验表明白藜芦醇和脂质纳米粒均未对Caco-2细胞单层的细胞活力和完整性产生不利影响。NLCs显著提高了白藜芦醇的体外肠道通透性，而SLNs并未损害白藜芦醇的吸收。由于在进食状态的肠液存在下肠道通透性增加，因此进餐期间白藜芦醇的口服吸收可得到增强。SLNs和NLCs构成了白藜芦醇口服给药的载体系统，可进一步用作补充剂或营养保健品。
