University of Veterinary Medicine Hannover, Institute of Food Toxicology and Analytical Chemistry, Bischofsholer Damm 15, 30173 Hannover, Germany.
University of Veterinary Medicine Hannover, Institute of Food Toxicology and Analytical Chemistry, Bischofsholer Damm 15, 30173 Hannover, Germany.
Food Chem. 2015 Jan 15;167:245-50. doi: 10.1016/j.foodchem.2014.06.103. Epub 2014 Jul 3.
Resveratrol oligomers are biologically active polyphenols found in wine. No information about the bioavailability of these polyphenols is available. In order to discover if the resveratrol oligomers can pass the intestinal barrier, transport of the dimer ε-viniferin and the tetramer hopeaphenol was studied in the Caco-2 transwell system. A flux through the cell monolayer could neither be observed for ε-viniferin nor for hopeaphenol (apparent permeability coefficient (Papp)<1×10(-6)cms(-1)). In contrast, resveratrol showed a Papp of 11.9×10(-6)cms(-1). Nevertheless, about 16-30% of the oligomers were found in the lysed cellular fraction. This leads to the conclusion that the intestinal absorption rate of the two resveratrol oligomers, ε-viniferin and hopeaphenol, is low and negligible when compared to resveratrol. Therefore, it is unlikely that the oligomers could elicit a systemic biological effect after dietary intake. However, the compounds may act locally on the intestinal epithelium.
白藜芦醇低聚体是存在于葡萄酒中的生物活性多酚。目前还没有关于这些多酚生物利用度的信息。为了发现白藜芦醇低聚体是否可以穿过肠屏障，在 Caco-2 跨膜系统中研究了二聚体 ε-viniferin 和四聚体 hopeaphenol 的转运。对于 ε-viniferin 和 hopeaphenol，既没有观察到穿过细胞单层的通量（表观渗透系数（Papp）<1×10(-6)cms(-1)）。相比之下，白藜芦醇的 Papp 为 11.9×10(-6)cms(-1)。然而，大约 16-30%的低聚体存在于裂解的细胞部分中。这得出结论，与白藜芦醇相比，两种白藜芦醇低聚体 ε-viniferin 和 hopeaphenol 的肠吸收速率较低且可以忽略不计。因此，膳食摄入后，这些低聚体不太可能在体内产生系统性的生物学效应。然而，这些化合物可能在肠上皮细胞局部发挥作用。
