Yang Yu, Wang Xiaoyu, Cui Qianling, Cao Qian, Li Lidong
School of Materials Science and Engineering, University of Science and Technology Beijing , Beijing 100083, China.
ACS Appl Mater Interfaces. 2016 Mar 23;8(11):7440-8. doi: 10.1021/acsami.6b00065. Epub 2016 Mar 14.
Fluorescent organic nanoparticles have attracted increasing attentions for chemical or biological sensing and imaging due to their low-toxicity, facile fabrication and surface functionalization. In this work, we report novel fluorescent organic nanoparticles via facile self-assembly method in aqueous solution. First, the designed water-soluble fluorophore shows a weak and negligible intrinsic fluorescence in water. Upon binding with adenosine-5'-triphosphate (ATP), fluorescent nanoparticles were formed immediately with strongly enhanced fluorescence. These fluorescent nanoparticles exhibit high sensitivity and selectivity toward Fe(3+) sensing with detection limit of 0.1 nM. In addition, after incubation with HeLa cells, the fluorophore shows excellent imaging performance by interaction with entogenous ATP in cells. Finally, this fluorescent system is also demonstrated to be capable of Fe(3+) sensing via fluorescence quenching in cellular environment.
荧光有机纳米颗粒因其低毒性、易于制备和表面功能化，在化学或生物传感与成像方面受到越来越多的关注。在这项工作中，我们报道了通过在水溶液中简便的自组装方法制备的新型荧光有机纳米颗粒。首先，设计的水溶性荧光团在水中显示出微弱且可忽略不计的固有荧光。与腺苷 - 5'-三磷酸（ATP）结合后，立即形成荧光纳米颗粒，荧光强度显著增强。这些荧光纳米颗粒对Fe(3+)传感具有高灵敏度和选择性，检测限为0.1 nM。此外，与HeLa细胞孵育后，荧光团通过与细胞内源性ATP相互作用表现出优异的成像性能。最后，该荧光系统还被证明能够在细胞环境中通过荧光猝灭实现对Fe(3+)的传感。
