铜绿假单胞菌中携带blaKPC-2的新型小质粒
New Small Plasmid Harboring blaKPC-2 in Pseudomonas aeruginosa.
作者信息
Galetti Renata, Andrade Leonardo Neves, Chandler Michael, Varani Alessandro de Mello, Darini Ana Lúcia Costa
机构信息
Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brazil
Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brazil.
出版信息
Antimicrob Agents Chemother. 2016 Apr 22;60(5):3211-4. doi: 10.1128/AAC.00247-16. Print 2016 May.
Abstract
The aim of this study was to characterize the genetic context of blaKPC-2 in Pseudomonas aeruginosa sequence type 244 from Brazil. The blaKPC-2 gene was detected in a new small plasmid, pBH6. Complete sequencing revealed that pBH6 was 3,652 bp long and included the Tn3 resolvase and Tn3 inverted repeat (IR), a partial copy of ISKpn6, and a putative ori region but no rep genes. pBH6 replicated stably into Escherichia coli strain DH10B and P. aeruginosa strain PAO.
摘要
本研究的目的是对来自巴西的244型铜绿假单胞菌中blaKPC-2的基因背景进行表征。在一个新的小质粒pBH6中检测到blaKPC-2基因。全序列测序显示，pBH6长3652 bp，包含Tn3解离酶和Tn3反向重复序列（IR）、ISKpn6的部分拷贝以及一个假定的ori区域，但没有复制基因。pBH6能在大肠杆菌DH10B菌株和铜绿假单胞菌PAO菌株中稳定复制。
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