Napoli Zaleida, Seghieri Giuseppe, Bianchi Loria, Anichini Roberto, De Bellis Alessandra, Campesi Ilaria, Carru Ciriaco, Occhioni Stefano, Zinellu Angelo, Franconi Flavia
Department of Clinical Chemistry, S. Jacopo Hospital, 51100 Pistoia, Italy.
Agenzia Regionale Sanità, Toscana, Florence, Italy; Accademia Medica Pistoiese F. Pacini, 51100 Pistoia, Italy.
J Diabetes Res. 2016;2016:7313162. doi: 10.1155/2016/7313162. Epub 2016 Feb 3.
Taurine transporter gene expression (RNA-TauT) has a role in retinal cell function and is modulated in vitro and in vivo by hyperglycemia and/or oxidative stress. This study was aimed at testing whether RNA-TauT gene expression is modified in blood mononuclear peripheral cells (MPCs) of type 1 diabetic patients, is related to plasma markers of oxidative stress or endothelial dysfunction, or, finally, is related to presence of retinopathy.
RNA-TauT was measured in MPCs by real-time PCR-analysis in 35 type 1 diabetic patients and in 33 age- and sex-matched controls, additionally measuring plasma and cell taurine and markers of oxidative stress and endothelial dysfunction.
RNA-TauT, expressed as 2(-ΔΔCt), was significantly higher in MPCs of type 1 diabetic patients than in controls [median (interquartile range): 1.32(0.31) versus 1.00(0.15); P = 0.01]. In diabetic patients RNA-TauT was related to HbA1c (r = 0.42; P = 0.01) and inversely to plasma homocysteine (r = -0.39; P = 0.02) being additionally significantly higher in MPCs of patients without retinopathy [(n = 22); 1.36(0.34)] compared to those with retinopathy [(n = 13); 1.16(0.20)], independently from HbA1c or diabetes duration.
RNA-TauT gene expression is significantly upregulated in MPCs of type 1 diabetes patients and is related to HbA1c levels and inversely to plasma homocysteine. Finally, in diabetes patients, RNA-TauT upregulation seems to be blunted in patients with retinopathy independently of their metabolic control or longer diabetes duration.
牛磺酸转运体基因表达（RNA-TauT）在视网膜细胞功能中发挥作用，且在体外和体内受高血糖和/或氧化应激调节。本研究旨在检测1型糖尿病患者外周血单个核细胞（MPCs）中RNA-TauT基因表达是否改变，是否与氧化应激或内皮功能障碍的血浆标志物相关，或者最终是否与视网膜病变的存在相关。
通过实时PCR分析在35例1型糖尿病患者和33例年龄及性别匹配的对照者的MPCs中测量RNA-TauT，另外测量血浆和细胞牛磺酸以及氧化应激和内皮功能障碍的标志物。
以2(-ΔΔCt)表示的RNA-TauT在1型糖尿病患者的MPCs中显著高于对照组[中位数（四分位间距）：1.32(0.31)对1.00(0.15)；P = 0.01]。在糖尿病患者中，RNA-TauT与糖化血红蛋白（HbA1c）相关（r = 0.42；P = 0.01），与血浆同型半胱氨酸呈负相关（r = -0.39；P = 0.02），此外，无视网膜病变患者（n = 22）的MPCs中RNA-TauT显著高于有视网膜病变患者（n = 13）[1.36(0.34)对1.16(0.20)]，独立于HbA1c或糖尿病病程。
1型糖尿病患者的MPCs中RNA-TauT基因表达显著上调，且与HbA1c水平相关，与血浆同型半胱氨酸呈负相关。最后，在糖尿病患者中，无论其代谢控制情况或糖尿病病程长短，视网膜病变患者的RNA-TauT上调似乎受到抑制。
