Zhou Sheng-fang, Liu Ru-xiu, Luo He-wei, Li Hui, Guan Xuan-ke, Yin Lin-lin, Li Li, Hu Dong-peng
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2016 Jan;36(1):85-9.
To observe the effect of Zishen Huoxue Recipe (ZHR) on pathomorphology in coronary heart disease (CHD) rats with Shen deficiency blood stasis syndrome (SDBSS).
Totally 60 healthy Wistar rats were divided into the blank control group, the model group, high, middle, and low dose ZHR groups according to random digit table, 12 in each group. Myocardial ischemia SDBSS rat model was prepared by ligating the left anterior descending coronary artery and injecting hydrocortisone. ZHR physic liquor was administered to rats in high, middle, and low dose ZHR groups at the daily dose of 21.6, 10.8, 5.4 g/kg by gastrogavage for 7 successive days, equal volume of pure water was administered to rats in the blank control group and the model group by gastrogavage for 7 successive days. Rat heart was collected for pathomorphological observation under light microscope.
In the model group the heart muscle fiber was swollen and deformed with widened space, loose and dropsy tissues. Blood vessels in myocardial mesenchymal were dilated, infiltrated with more inflammatory cells. Myocardial cells were markedly swollen, degenerated, or necrotic, with caryolysis or disappearance of partial nuclear. A large amount of collagen fibrous tissue became hyperplasia. Endocardial blood vessels were swollen and degenerated with infiltration of few inflammatory cells. Epicardium tissue and structure were destroyed and got hyperplasia. Swollen, degenerated, or necrotic vessels could be seen, with infiltration of more inflammatory cells and collagen deposition. Pathomorphological injuries were alleviated in each ZHR group. The higher ZHR concentration, the milder the injury degree of myocardial tissue, the more limited range of damage.
ZHR could attenuate pathomorphological injuries of myocardial ischemia rats with SDBSS and regulate myocardial function, thus improving myocardial ischemia in CHD rats with SDBSS.
观察滋肾活血方对冠心病肾虚血瘀证大鼠病理形态学的影响。
将60只健康Wistar大鼠按随机数字表法分为空白对照组、模型组、滋肾活血方高、中、低剂量组，每组12只。采用结扎冠状动脉左前降支并注射氢化可的松的方法制备心肌缺血肾虚血瘀证大鼠模型。滋肾活血方高、中、低剂量组大鼠分别按每日21.6、10.8、5.4 g/kg的剂量灌胃给予滋肾活血方药液，连续7天；空白对照组和模型组大鼠按等体积灌胃给予纯净水，连续7天。取大鼠心脏进行光镜下病理形态学观察。
模型组心肌纤维肿胀变形，间隙增宽，组织疏松、水肿。心肌间质血管扩张，有较多炎性细胞浸润。心肌细胞明显肿胀、变性或坏死，部分细胞核溶解或消失。大量胶原纤维组织增生。心内膜血管肿胀、变性，有少量炎性细胞浸润。心外膜组织结构破坏、增生。可见血管肿胀、变性或坏死，有较多炎性细胞浸润及胶原沉积。各滋肾活血方组病理形态学损伤均减轻。滋肾活血方浓度越高，心肌组织损伤程度越轻，损伤范围越局限。
滋肾活血方可减轻冠心病肾虚血瘀证心肌缺血大鼠的病理形态学损伤，调节心肌功能，从而改善冠心病肾虚血瘀证大鼠的心肌缺血情况。
