使用催化化学交换饱和转移（CatalyCEST）磁共振成像造影剂检测硫酸酯酶活性
Detection of Sulfatase Enzyme Activity with a CatalyCEST MRI Contrast Agent.
作者信息
Sinharay Sanhita, Fernández-Cuervo Gabriela, Acfalle Jasmine P, Pagel Mark D
机构信息
Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, USA.
Department of Pharmaceutical Sciences, University of Arizona, Tucson, AZ, USA.
出版信息
Chemistry. 2016 May 4;22(19):6491-5. doi: 10.1002/chem.201600685. Epub 2016 Mar 23.
Abstract
A chemical exchange saturation transfer (CEST) MRI contrast agent has been developed that detects sulfatase enzyme activity. The agent produces a CEST signal at δ=5.0 ppm before enzyme activity, and a second CEST signal appears at δ=9.0 ppm after the enzyme cleaves a sulfate group from the agent. The comparison of the two signals improved detection of sulfatase activity.
摘要
一种用于检测硫酸酯酶活性的化学交换饱和转移（CEST）磁共振成像造影剂已被研发出来。该造影剂在酶活性发挥作用之前，于δ=5.0 ppm处产生一个CEST信号，而在酶从造影剂上裂解掉一个硫酸基团之后，会在δ=9.0 ppm处出现第二个CEST信号。这两个信号的比较提高了对硫酸酯酶活性的检测能力。
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