Margiotta D, Navarini L, Vadacca M, Basta F, Lo Vullo M, Pignataro F, Zardi E M, Afeltra A
Clinical Medicine and Rheumatology Department, Campus Bio-Medico University of Rome, Rome, Italy.
Eur Rev Med Pharmacol Sci. 2016;20(4):636-41.
Crescent literature data demonstrated a role of adipokines in immune responses, particularly leptin is involved in wide spectrum of pro-inflammatory functions. Several evidences suggested that leptin is able to inhibit T regulatory cells proliferation and function in vitro models. In the present study, we investigate the relationship between leptin and circulating T regulatory cells (Tregs) in patients affected by systemic lupus erythematosus (SLE).
13 SLE patients and 11 healthy controls were enrolled. Metabolic syndrome and cardiovascular parameters were evaluated. Serum leptin levels were detected by commercial ELISA kit and circulating regulatory T cells were determined by FACS analysis as CD4+CD25highFOP3+ lymphocytes.
Metabolic syndrome, defined by ATPIII criteria, was more prevalent in SLE compared to controls (38.4% vs. 0%, p = 0.04), as well as arterial hypertension (38.4% vs. 0%, p = 0.04). We did not find significant differences in mean leptin levels among SLE and controls (13.13 ± 1.51 ng/ml vs. 9.48 ± 8.67 ng/ml, p = 0.6). Mean Tregs percentage of total CD4 were 1.27 ± 0.9 in SLE vs. 2.8 ± 1.2 in healthy controls (p = 0.001). We found a negative correlation between leptin levels and Tregs percentage of total CD4 in SLE patients (r = 0.4, p = 0.01).
Our results suggest a role of leptin in the regulation of circulating T regulatory cells amount in human SLE.
新月形文献数据表明脂肪因子在免疫反应中发挥作用，尤其是瘦素参与了广泛的促炎功能。多项证据表明，在体外模型中瘦素能够抑制调节性T细胞的增殖和功能。在本研究中，我们调查了系统性红斑狼疮（SLE）患者中瘦素与循环调节性T细胞（Tregs）之间的关系。
招募了13例SLE患者和11名健康对照者。评估代谢综合征和心血管参数。采用商用ELISA试剂盒检测血清瘦素水平，通过流式细胞术分析将循环调节性T细胞测定为CD4+CD25highFOP3+淋巴细胞。
根据ATPIII标准定义的代谢综合征在SLE患者中比对照组更普遍（38.4%对0%，p = 0.04），动脉高血压也是如此（38.4%对0%，p = 0.04）。我们发现SLE患者和对照组之间的平均瘦素水平没有显著差异（13.13±1.51 ng/ml对9.48±8.67 ng/ml，p = 0.6）。SLE患者中CD4总数的平均Tregs百分比为1.27±0.9，而健康对照组为2.8±1.2（p = 0.001）。我们发现SLE患者中瘦素水平与CD4总数的Tregs百分比之间存在负相关（r = 0.4，p = 0.01）。
我们的结果表明瘦素在调节人类SLE中循环调节性T细胞数量方面发挥作用。
