Wandersman C
Unité de Génétique Moléculaire, Institute Pasteur, Paris, France.
Mol Microbiol. 1989 Dec;3(12):1825-31. doi: 10.1111/j.1365-2958.1989.tb00169.x.
Many different bacteria secrete proteases into the culture medium. Extracellular proteases produced by Gram-positive bacteria are secreted by a signal-peptide-dependent pathway and have a propeptide located between the signal peptide and the mature protein. Many extracellular proteases synthesized by Gram-negative bacteria are also produced as precursors with a signal peptide. However, at least two species of Gram-negative bacteria secrete one or more proteases via a novel signal-peptide-independent route. Most proteases secreted by Gram-negative bacteria also have a propeptide whose length and location vary according to the protease. Specific features of protease secretion pathways and the mechanisms of protease activation are discussed with particular reference to some of the best-characterized extracellular proteases produced by Gram-positive and Gram-negative bacteria.
许多不同的细菌会向培养基中分泌蛋白酶。革兰氏阳性菌产生的细胞外蛋白酶通过信号肽依赖性途径分泌，并且在信号肽和成熟蛋白之间有一个前肽。革兰氏阴性菌合成的许多细胞外蛋白酶也以前体形式产生，带有信号肽。然而，至少有两种革兰氏阴性菌通过一种新的不依赖信号肽的途径分泌一种或多种蛋白酶。革兰氏阴性菌分泌的大多数蛋白酶也有一个前肽，其长度和位置因蛋白酶而异。本文将特别参考革兰氏阳性菌和革兰氏阴性菌产生的一些特征明确的细胞外蛋白酶，讨论蛋白酶分泌途径的具体特点以及蛋白酶激活的机制。
