Osadchaia A I, Podgorskiĭ V S, Prokopchenko S F, Mikhnovskaia N D, Vasil'evskaia I A
Mikrobiol Zh (1978). 1989 Nov-Dec;51(6):45-50.
The method of inoculation was studied for its effect on the growth and variability of Bacillus thuringiensis H14 266/2-1 when cultivating them in the liquid nutrient medium inoculate with inoculum prepared by different procedures. Optimal conditions of the inoculum preparation are determined. Productivity of the strain when inoculating the medium with the aerial-dry inoculum was studied as compared to the inoculation by the inoculum taken from the mown agar. It is established that selection of the virulent R-form from colonies should serve as a basis for the preparation of the stable morphologically uniform inoculum. The aerial-dried culture of bacilli is shown to be more effective since it is characterized by higher and stable indices of productivity and is more prepared to a long-duration storage than the inoculated agar media. A more rapid transition (by 2-4h) of the culture to the use of the carbon source is marked. The culture of bacilli is different in a higher developmental level: sporulation and crystallization initiate during the first hours of inoculation and by the 24th hour sporulation reaches 100% with the prevailing content of R-form.
研究了接种方法对苏云金芽孢杆菌H14 266/2-1在液体营养培养基中生长和变异性的影响，该培养基接种了通过不同程序制备的接种物。确定了接种物制备的最佳条件。研究了与取自割下琼脂的接种物接种相比，用风干接种物接种培养基时菌株的生产力。确定从菌落中选择有毒力的R型应作为制备稳定的形态均匀接种物的基础。杆菌的风干培养物显示出更高的效率，因为它具有更高且稳定的生产力指标，并且比接种的琼脂培养基更易于长期保存。标记出培养物向使用碳源的转变更快（提前2-4小时）。杆菌培养物在更高的发育水平上有所不同：接种后的最初几个小时内开始产孢和结晶，到第24小时产孢率达到100%，且R型占主导。
